Rapid immunoglobulin M-based dengue diagnostic test using surface plasmon resonance biosensor

Peyman Jahanshahi; Erfan Zalnezhad; Shamala Devi Sekaran; Faisal Rafiq Mahamd Adikan

doi:10.1038/srep03851

Rapid immunoglobulin M-based dengue diagnostic test using surface plasmon resonance biosensor

Peyman Jahanshahi, Erfan Zalnezhad, Shamala Devi Sekaran, Faisal Rafiq Mahamd Adikan

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

Surface plasmon resonance (SPR) is a medical diagnosis technique with high sensitivity and specificity. In this research, a new method based on SPR is proposed for rapid, 10-minute detection of the anti-dengue virus in human serum samples. This novel technique, known as rapid immunoglobulin M (IgM)-based dengue diagnostic test, can be utilized quickly and easily at the point of care. Four dengue virus serotypes were used as ligands on a biochip. According to the results, a serum volume of only 1â€...μl from a dengue patient (as a minimized volume) is required to indicate SPR angle variation to determine the ratio of each dengue serotype in samples with 83-93% sensitivity and 100% specificity.

Original language	English
Article number	3851
Journal	Scientific Reports
Volume	4
DOIs	https://doi.org/10.1038/srep03851
Publication status	Published - 24 Jan 2014
Externally published	Yes

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abstract = "Surface plasmon resonance (SPR) is a medical diagnosis technique with high sensitivity and specificity. In this research, a new method based on SPR is proposed for rapid, 10-minute detection of the anti-dengue virus in human serum samples. This novel technique, known as rapid immunoglobulin M (IgM)-based dengue diagnostic test, can be utilized quickly and easily at the point of care. Four dengue virus serotypes were used as ligands on a biochip. According to the results, a serum volume of only 1{\^a}€...μl from a dengue patient (as a minimized volume) is required to indicate SPR angle variation to determine the ratio of each dengue serotype in samples with 83-93% sensitivity and 100% specificity.",

author = "Peyman Jahanshahi and Erfan Zalnezhad and Sekaran, {Shamala Devi} and Adikan, {Faisal Rafiq Mahamd}",

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AU - Zalnezhad, Erfan

AU - Sekaran, Shamala Devi

AU - Adikan, Faisal Rafiq Mahamd

PY - 2014/1/24

Y1 - 2014/1/24

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Rapid immunoglobulin M-based dengue diagnostic test using surface plasmon resonance biosensor

Abstract

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