Rapid determination of protein contents in microencapsulated fish oil supplements by ATR-FTIR spectroscopy and Partial Least Square Regression (PLSR) analysis

Jitraporn Vongsvivut, Philip Robert Heraud, Wei Zhang, Jaroslav A Kralovec, Donald McNaughton, Colin James Barrow

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Following the recent success in quantitative analysis of essential fatty acid compositions in a commercial microencapsulated fish oil (?EFO) supplement, we extended the application of portable attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic technique and partial least square regression (PLSR) analysis for rapid determination of total protein contents-the other major component in most commercial ?EFO powders. In contrast to the traditional chromatographic methodology used in a routine amino acid analysis (AAA), the ATR-FTIR spectra of the ?EFO powder can be acquired directly from its original powder form with no requirement of any sample preparation, making the technique exceptionally fast, noninvasive, and environmentally friendly as well as being cost effective and hence eminently suitable for routine use by industry.
Original languageEnglish
Pages (from-to)265 - 277
Number of pages13
JournalFood and Bioprocess Technology
Volume7
Issue number1
DOIs
Publication statusPublished - 2014

Cite this

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title = "Rapid determination of protein contents in microencapsulated fish oil supplements by ATR-FTIR spectroscopy and Partial Least Square Regression (PLSR) analysis",
abstract = "Following the recent success in quantitative analysis of essential fatty acid compositions in a commercial microencapsulated fish oil (?EFO) supplement, we extended the application of portable attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic technique and partial least square regression (PLSR) analysis for rapid determination of total protein contents-the other major component in most commercial ?EFO powders. In contrast to the traditional chromatographic methodology used in a routine amino acid analysis (AAA), the ATR-FTIR spectra of the ?EFO powder can be acquired directly from its original powder form with no requirement of any sample preparation, making the technique exceptionally fast, noninvasive, and environmentally friendly as well as being cost effective and hence eminently suitable for routine use by industry.",
author = "Jitraporn Vongsvivut and Heraud, {Philip Robert} and Wei Zhang and Kralovec, {Jaroslav A} and Donald McNaughton and Barrow, {Colin James}",
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Rapid determination of protein contents in microencapsulated fish oil supplements by ATR-FTIR spectroscopy and Partial Least Square Regression (PLSR) analysis. / Vongsvivut, Jitraporn; Heraud, Philip Robert; Zhang, Wei; Kralovec, Jaroslav A; McNaughton, Donald; Barrow, Colin James.

In: Food and Bioprocess Technology, Vol. 7, No. 1, 2014, p. 265 - 277.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Rapid determination of protein contents in microencapsulated fish oil supplements by ATR-FTIR spectroscopy and Partial Least Square Regression (PLSR) analysis

AU - Vongsvivut, Jitraporn

AU - Heraud, Philip Robert

AU - Zhang, Wei

AU - Kralovec, Jaroslav A

AU - McNaughton, Donald

AU - Barrow, Colin James

PY - 2014

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AB - Following the recent success in quantitative analysis of essential fatty acid compositions in a commercial microencapsulated fish oil (?EFO) supplement, we extended the application of portable attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopic technique and partial least square regression (PLSR) analysis for rapid determination of total protein contents-the other major component in most commercial ?EFO powders. In contrast to the traditional chromatographic methodology used in a routine amino acid analysis (AAA), the ATR-FTIR spectra of the ?EFO powder can be acquired directly from its original powder form with no requirement of any sample preparation, making the technique exceptionally fast, noninvasive, and environmentally friendly as well as being cost effective and hence eminently suitable for routine use by industry.

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DO - 10.1007/s11947-013-1122-8

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JO - Food and Bioprocess Technology

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SN - 1935-5130

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