Projects per year
Abstract
Novel doping agents and doping strategies are continually entering the market, placing a burden on analytical methods to detect, adapt, and respond to subtle changes in the composition of biological samples. Therefore, there is a growing interest in rapid, adaptable, and ideally confirmatory analytical methods for the fight against doping. Nanostructured silicon (nano-Si)-based surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) can effectively address this need, allowing fast and sensitive detection of prohibited compounds used in sport doping. Here, we demonstrate the detection of growth hormone peptides, anabolic-androgenic steroids, and narcotics at low concentrations directly from biological matrices. Molecular confirmation was performed using the fragmentation data of the structures, obtained with the tandem mass spectrometry capabilities of the SALDI instrument. The obtained data were in excellent agreement with those obtained using leading triple quadrupole liquid chromatography-mass spectrometry instruments. Furthermore, nano-Si SALDI-MS has the capacity for high-throughput analysis of hundreds of biological samples, providing opportunities for real-time MS analysis at sporting events.
Original language | English |
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Pages (from-to) | 31195-31204 |
Number of pages | 10 |
Journal | ACS Applied Materials & Interfaces |
Volume | 12 |
Issue number | 28 |
DOIs | |
Publication status | Published - 15 Jul 2020 |
Keywords
- detection
- DIOS
- doping
- mass spectrometry
- NIMS
- rapid
- SALDI-MS
Projects
- 1 Finished
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Lab-on-a-chip mass spectrometry tools for testing illicit drugs
Voelcker, N., Kirkbride, K., Nussio, M. & Stockham, P.
1/02/16 → 31/12/19
Project: Research
Equipment
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Melbourne Centre for Nanofabrication
Sean Langelier (Manager)
Office of the Vice-Provost (Research and Research Infrastructure)Facility/equipment: Facility