Rapid and simple high-performance liquid chromatographic assay for the determination of metformin in human plasma and breast milk

Mei Zhang; Grant A. Moore; Michael Lever; Sharon J. Gardiner; Carl M.J. Kirkpatrick; Evan J. Begg

doi:10.1016/S0378-4347(01)00430-3

Rapid and simple high-performance liquid chromatographic assay for the determination of metformin in human plasma and breast milk

Mei Zhang, Grant A. Moore, Michael Lever, Sharon J. Gardiner, Carl M.J. Kirkpatrick, Evan J. Begg

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

A rapid and simple high-performance liquid chromatographic (HPLC) assay for the determination of metformin in human plasma and breast milk is described. After proteins were precipitated with acetonitrile, metformin and the internal standard buformin were resolved on a cation-exchange column and detected by UV detection at 236 nm. Standard curves were linear over the concentration range 20.0-4000 μg/l. Intra- and inter-day coefficients of variation were <9.0% and the limit of quantification was around 20 μg/l.

Original language	English
Pages (from-to)	175-179
Number of pages	5
Journal	Journal of Chromatography B
Volume	766
Issue number	1
DOIs	https://doi.org/10.1016/S0378-4347(01)00430-3
Publication status	Published - 1 Jan 2002
Externally published	Yes

Keywords

Metformin

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10.1016/S0378-4347(01)00430-3

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abstract = "A rapid and simple high-performance liquid chromatographic (HPLC) assay for the determination of metformin in human plasma and breast milk is described. After proteins were precipitated with acetonitrile, metformin and the internal standard buformin were resolved on a cation-exchange column and detected by UV detection at 236 nm. Standard curves were linear over the concentration range 20.0-4000 μg/l. Intra- and inter-day coefficients of variation were <9.0% and the limit of quantification was around 20 μg/l.",

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AU - Moore, Grant A.

AU - Lever, Michael

AU - Gardiner, Sharon J.

AU - Kirkpatrick, Carl M.J.

AU - Begg, Evan J.

PY - 2002/1/1

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AB - A rapid and simple high-performance liquid chromatographic (HPLC) assay for the determination of metformin in human plasma and breast milk is described. After proteins were precipitated with acetonitrile, metformin and the internal standard buformin were resolved on a cation-exchange column and detected by UV detection at 236 nm. Standard curves were linear over the concentration range 20.0-4000 μg/l. Intra- and inter-day coefficients of variation were <9.0% and the limit of quantification was around 20 μg/l.

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IS - 1

ER -

Rapid and simple high-performance liquid chromatographic assay for the determination of metformin in human plasma and breast milk

Abstract

Keywords

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