TY - JOUR
T1 - Rapid and Selective Biomarker Detection with Conductometric Sensors
AU - Perera, Ganganath S.
AU - Ahmed, Taimur
AU - Heiss, Leah
AU - Walia, Sumeet
AU - Bhaskaran, Madhu
AU - Sriram, Sharath
N1 - Funding Information:
This work was performed in part at the Micro Nano Research Facility at RMIT University in the Victorian Node of the Australian National Fabrication Facility (ANFF). The authors also acknowledge Dustin Bailey for the contribution in preparation of illustrations of antibody?antigen interactions on GPS functionalized-devices. The authors are especially thankful to Moritz Doering from Functional Materials and Microsystems Research Group for contributing to developing measurement protocols in the early stages of this study.
Publisher Copyright:
© 2021 Wiley-VCH GmbH
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
MDTHM_HHE
PY - 2021/2/18
Y1 - 2021/2/18
N2 - The biomarker detection in human body fluids is crucial as biomarkers are important in diagnosing diseases. Conventional invasive techniques for biomarker detection are associated with infection, tissue damage, and discomfort. Non-invasive devices are an attractive alternative. Here, metal oxide (oxygen-deficient zinc oxide, ZnO) based conductometric sensors with two-terminal electrodes for rapid detection of biomarkers in real-time, are presented. This platform can be engineered for non-invasive, sensitive, and on-demand selective detection of biomarkers based on surface functionalization. The three novelties in this biosensing technique include an on‑demand target selection device platform, short (<10 min) incubation times, and real-time monitoring of the biomarker of interest by electrical (resistance change) measurements. Cardiac inflammatory biomarkers interleukin 6 (IL-6) and C-reactive protein (CRP) are used as the model antigens. The devices can detect 100× lower concentration of IL-6 than healthy levels in human saliva and sweat and 1000× and ≈50× lower CRP concentrations than healthy levels in human saliva and sweat, respectively. The devices show high selectivity for IL-6 and CRP antigens when tested with a mixture of biomarkers. This sensor platform can be extended to selective measurements for viruses or DNA screening, which enables a new category of compact and rapid point-of-care medical devices.
AB - The biomarker detection in human body fluids is crucial as biomarkers are important in diagnosing diseases. Conventional invasive techniques for biomarker detection are associated with infection, tissue damage, and discomfort. Non-invasive devices are an attractive alternative. Here, metal oxide (oxygen-deficient zinc oxide, ZnO) based conductometric sensors with two-terminal electrodes for rapid detection of biomarkers in real-time, are presented. This platform can be engineered for non-invasive, sensitive, and on-demand selective detection of biomarkers based on surface functionalization. The three novelties in this biosensing technique include an on‑demand target selection device platform, short (<10 min) incubation times, and real-time monitoring of the biomarker of interest by electrical (resistance change) measurements. Cardiac inflammatory biomarkers interleukin 6 (IL-6) and C-reactive protein (CRP) are used as the model antigens. The devices can detect 100× lower concentration of IL-6 than healthy levels in human saliva and sweat and 1000× and ≈50× lower CRP concentrations than healthy levels in human saliva and sweat, respectively. The devices show high selectivity for IL-6 and CRP antigens when tested with a mixture of biomarkers. This sensor platform can be extended to selective measurements for viruses or DNA screening, which enables a new category of compact and rapid point-of-care medical devices.
KW - antibodies
KW - biosensors
KW - oxides
KW - thin films
UR - http://www.scopus.com/inward/record.url?scp=85099846200&partnerID=8YFLogxK
U2 - 10.1002/smll.202005582
DO - 10.1002/smll.202005582
M3 - Article
C2 - 33502115
AN - SCOPUS:85099846200
SN - 1613-6810
VL - 17
JO - Small
JF - Small
IS - 7
M1 - 2005582
ER -