TY - JOUR
T1 - Raman spectroscopy analysis of lipid droplets content, distribution and saturation level in Non-Alcoholic Fatty Liver Disease in mice
AU - Kochan, Kamila
AU - Maslak, Edyta
AU - Krafft, Christoph
AU - Kostogrys, Renata
AU - Chlopicki, Stefan
AU - Baranska, Malgorzata
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Non-Alcoholic Fatty Liver Disease (NAFLD) is a common liver disorder, characterized by an excessive lipids deposition within the hepatic tissue. Due to the lack of clear-cut symptoms and optimal diagnostic method, the actual prevalence of NAFLD and its pathogenesis remains unclear, especially in the early stages of progression. In the presented work confocal Raman microspectroscopy was used to investigate alterations in the chemical composition of the NAFLD-affected liver. We have investigated two NAFLD models, representative for macrovesicular and microvesicular steatosis, induced by High Fat Diet (60 kcal %) and Low Carbohydrate High Protein Diet (LCHP), respectively. In both models we confirmed the development of NAFLD, manifested by the presence of lipid droplets (LDs), but of different sizes. Model of macrovesicular steatosis was characterized by large LDs, whereas in the microvesicular steatosis model small droplets were found. In both models, however, we observed a significant decrease in the degree of unsaturation of lipids, in comparison to the control. In addition, for both models, the impact of medical treatment with selected drugs (perindopril and nicotinic acid, respectively) was tested, indicating a significant influence of medicine not only on the occurrence and size of the droplets, but also on their composition. Inboth cases the drug treatment resulted in an increase of the degree of unsaturation of lipids forming droplets. Confocal Raman microspectroscopy was proven to be a powerful tool providing detailed insight into selected areas of hepatic tissue, following the NAFLD pathogenesis and diagnostic potential of the applied drugs. This work presents a confocal Raman microspectroscopic study of the alterations in the composition of NAFLD-affected liver. We confirm the NAFLD development, manifested by the presence of lipid droplets of different sizes, in models of macrovesicular and microvesicular steatosis. In both we observe a significant decrease in the degree of unsaturation of lipids, compared to control. These changes are reversed in the initial stage of medical treatment.
AB - Non-Alcoholic Fatty Liver Disease (NAFLD) is a common liver disorder, characterized by an excessive lipids deposition within the hepatic tissue. Due to the lack of clear-cut symptoms and optimal diagnostic method, the actual prevalence of NAFLD and its pathogenesis remains unclear, especially in the early stages of progression. In the presented work confocal Raman microspectroscopy was used to investigate alterations in the chemical composition of the NAFLD-affected liver. We have investigated two NAFLD models, representative for macrovesicular and microvesicular steatosis, induced by High Fat Diet (60 kcal %) and Low Carbohydrate High Protein Diet (LCHP), respectively. In both models we confirmed the development of NAFLD, manifested by the presence of lipid droplets (LDs), but of different sizes. Model of macrovesicular steatosis was characterized by large LDs, whereas in the microvesicular steatosis model small droplets were found. In both models, however, we observed a significant decrease in the degree of unsaturation of lipids, in comparison to the control. In addition, for both models, the impact of medical treatment with selected drugs (perindopril and nicotinic acid, respectively) was tested, indicating a significant influence of medicine not only on the occurrence and size of the droplets, but also on their composition. Inboth cases the drug treatment resulted in an increase of the degree of unsaturation of lipids forming droplets. Confocal Raman microspectroscopy was proven to be a powerful tool providing detailed insight into selected areas of hepatic tissue, following the NAFLD pathogenesis and diagnostic potential of the applied drugs. This work presents a confocal Raman microspectroscopic study of the alterations in the composition of NAFLD-affected liver. We confirm the NAFLD development, manifested by the presence of lipid droplets of different sizes, in models of macrovesicular and microvesicular steatosis. In both we observe a significant decrease in the degree of unsaturation of lipids, compared to control. These changes are reversed in the initial stage of medical treatment.
KW - KMC
KW - Lipid droplets
KW - Liver
KW - NAFLD
KW - Raman mapping
UR - http://www.scopus.com/inward/record.url?scp=84933527304&partnerID=8YFLogxK
U2 - 10.1002/jbio.201400077
DO - 10.1002/jbio.201400077
M3 - Article
AN - SCOPUS:84933527304
SN - 1864-063X
VL - 8
SP - 597
EP - 609
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 7
ER -