Raman imaging of biomedical samples

Agnieszka Kaczor, Katarzyna M. Marzec, Katarzyna Majzner, Kamila Kochan, Marta Z. Pacia, Malgorzata Baranska

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

3 Citations (Scopus)

Abstract

Fluorescence microscopy, a gold standard in the tissue or cell imaging, has several drawbacks, with a severe one being its disability to elucidate the chemical characteristics of the sample. Confocal Raman microscopy is a tempting label free alternative to fluorescence and a prospective future method of medical diagnostics. Raman measurements of tissues can provide information about their chemical composition, i.e. main components but also specific compounds, and their changes upon the development of patholog or its treatment. The analysis is based on characteristic marker bands, provided they can be identified in the spectra, or by more common approach using chemometrics. Moreover, Raman microscopy can be used to detect small biochemical alterations and their respective distribution in a single cell or even at sub-cellular level. The impact of various factors, including the uptake of drugs, bioactive compounds or non-chemical stressors can be observed using such investigations of cells.

Original languageEnglish
Title of host publicationConfocal Raman Microscopy
EditorsJan Toporski, Thomas Dieing, Olaf Hollricher
Place of PublicationCham Switzerland
PublisherSpringer
Chapter14
Pages307-346
Number of pages40
Edition2nd
ISBN (Electronic)9783319753805
ISBN (Print)9783319753782
DOIs
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameSpringer Series in Surface Sciences
Volume66
ISSN (Print)0931-5195
ISSN (Electronic)2198-4743

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