RAFT polymerzation for delivery of bioactives

J. Chiefari; C. Guerrero-Sanchez; G. Moad; S. H. Thang; J. Tsanaktsidis; Thilak Gunatillake; T. Hinton; S. Shi; M. Tizard

RAFT polymerzation for delivery of bioactives

J. Chiefari, C. Guerrero-Sanchez, G. Moad, S. H. Thang, J. Tsanaktsidis, Thilak Gunatillake, T. Hinton, S. Shi, M. Tizard

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research

Abstract

This paper describes recent efforts on the use of Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerization for synthesis of well-defined polymer conjugates for delivery of bioactives. The research is illustrated by work on with the antitumor drug SN38, to improve water solubility and cell viability, and the delivery of siRNA, for gene silencing. The RAFT polymers are conjugated to the active through a covalent linkage, in the case of anticancer drug SN38, and by ionic association, for the case of siRNA. Preliminary results are extremely encouraging. The synthesized RAFT ABA triblock polymers provided 100% siRNA uptake and gave gene silencing similar to that of the transfection reagent Lipofectamine™. In both examples RAFT-acive conjugates polymers were well-tolerated showing no discernable cell cytotoxicity.

Original language	English
Title of host publication	Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Subtitle of host publication	Boston, MA, United States; 13-16 June 2011
Pages	186-188
Number of pages	3
Volume	3
Publication status	Published - 23 Nov 2011
Externally published	Yes

Keywords

Bioconjuates
RAFT polymerization
siRNA
SN38 antitumor drug

Cite this

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title = "RAFT polymerzation for delivery of bioactives",

abstract = "This paper describes recent efforts on the use of Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerization for synthesis of well-defined polymer conjugates for delivery of bioactives. The research is illustrated by work on with the antitumor drug SN38, to improve water solubility and cell viability, and the delivery of siRNA, for gene silencing. The RAFT polymers are conjugated to the active through a covalent linkage, in the case of anticancer drug SN38, and by ionic association, for the case of siRNA. Preliminary results are extremely encouraging. The synthesized RAFT ABA triblock polymers provided 100% siRNA uptake and gave gene silencing similar to that of the transfection reagent Lipofectamine{\texttrademark}. In both examples RAFT-acive conjugates polymers were well-tolerated showing no discernable cell cytotoxicity.",

keywords = "Bioconjuates, RAFT polymerization, siRNA, SN38 antitumor drug",

author = "J. Chiefari and C. Guerrero-Sanchez and G. Moad and Thang, {S. H.} and J. Tsanaktsidis and Thilak Gunatillake and T. Hinton and S. Shi and M. Tizard",

year = "2011",

month = nov,

day = "23",

language = "English",

isbn = "9781439871386",

volume = "3",

pages = "186--188",

booktitle = "Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011",

}

RAFT polymerzation for delivery of bioactives. / Chiefari, J.; Guerrero-Sanchez, C.; Moad, G.; Thang, S. H.; Tsanaktsidis, J.; Gunatillake, Thilak; Hinton, T.; Shi, S.; Tizard, M.

Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011: Boston, MA, United States; 13-16 June 2011. Vol. 3 2011. p. 186-188.

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research

TY - GEN

T1 - RAFT polymerzation for delivery of bioactives

AU - Chiefari, J.

AU - Guerrero-Sanchez, C.

AU - Moad, G.

AU - Thang, S. H.

AU - Tsanaktsidis, J.

AU - Gunatillake, Thilak

AU - Hinton, T.

AU - Shi, S.

AU - Tizard, M.

PY - 2011/11/23

Y1 - 2011/11/23

N2 - This paper describes recent efforts on the use of Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerization for synthesis of well-defined polymer conjugates for delivery of bioactives. The research is illustrated by work on with the antitumor drug SN38, to improve water solubility and cell viability, and the delivery of siRNA, for gene silencing. The RAFT polymers are conjugated to the active through a covalent linkage, in the case of anticancer drug SN38, and by ionic association, for the case of siRNA. Preliminary results are extremely encouraging. The synthesized RAFT ABA triblock polymers provided 100% siRNA uptake and gave gene silencing similar to that of the transfection reagent Lipofectamine™. In both examples RAFT-acive conjugates polymers were well-tolerated showing no discernable cell cytotoxicity.

AB - This paper describes recent efforts on the use of Reversible Addition-Fragmentation Chain Transfer (RAFT) polymerization for synthesis of well-defined polymer conjugates for delivery of bioactives. The research is illustrated by work on with the antitumor drug SN38, to improve water solubility and cell viability, and the delivery of siRNA, for gene silencing. The RAFT polymers are conjugated to the active through a covalent linkage, in the case of anticancer drug SN38, and by ionic association, for the case of siRNA. Preliminary results are extremely encouraging. The synthesized RAFT ABA triblock polymers provided 100% siRNA uptake and gave gene silencing similar to that of the transfection reagent Lipofectamine™. In both examples RAFT-acive conjugates polymers were well-tolerated showing no discernable cell cytotoxicity.

KW - Bioconjuates

KW - RAFT polymerization

KW - siRNA

KW - SN38 antitumor drug

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M3 - Conference Paper

AN - SCOPUS:81455139934

SN - 9781439871386

VL - 3

SP - 186

EP - 188

BT - Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011

ER -

RAFT polymerzation for delivery of bioactives

Abstract

Keywords

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Cite this