Quinazolinone exposure induces apoptosis and changes expressions of Fas/FasL and C-Flip in embryonic mice testicles

Maryam Shams Lahijani, Shirin Farivar, Mojgan Sarhady, Mitra Amiri

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Abstract

Quinazolinones represent a class of sedative and anticancer drugs. Quinazolinones-based compounds have ability to suppress prostate tumor growth via apoptosis. Apoptosis is very common in embryos and adults of normal and injured mammalian testes. Effects a new derivative of quinazolinone (4(3H) quinazolinone-2-ethyl-2-phenyl ethyl (QEPE)), on the testis of Balb/C mice embryos were investigated. QEPE was able to reduce number of germ cells and diameter of seminiferous tubules. TUNEL assay analysis indicated that reduction correlated with an increase in the number of apoptotic cell. Furthermore, electron microscope observations confirmed typical apoptotic morphologies characterized by chromatin fragmentation. Finally, RT-PCR analysis showed QEPE increases the levels of Fas/Fasl and decreases C-Flip mRNAs in the testis of exposed embryos.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalIndian Journal of Experimental Biology
Volume50
Issue number4
Publication statusPublished - 1 Apr 2012
Externally publishedYes

Keywords

  • Apoptosis
  • C-flip
  • Embryonic testis
  • Fas/fasl
  • Quinazolinones

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