Quantum dot-antisense oligonucleotide conjugates for multifunctional gene transfection, mRNA regulation, and tracking of biological processes

Yilin  Li; Xin Duan; Lihong Jing; Chunhui Yang; Ruirui Qiao; Mingyuan Gao

doi:10.1016/j.biomaterials.2010.11.024

Quantum dot-antisense oligonucleotide conjugates for multifunctional gene transfection, mRNA regulation, and tracking of biological processes

Yilin Li, Xin Duan, Lihong Jing, Chunhui Yang, Ruirui Qiao, Mingyuan Gao

Research output: Contribution to journal › Article › Research › peer-review

45 Citations (Scopus)

Abstract

It was demonstrated that oligonucleotides, independent of their base sequence and length, could effectively induce the cellular uptake of mercapto acid-capped CdTe QDs after the oligonucleotides were covalently attached on the surface of the QDs. Following these experimental observations, a conjugate composed of covalently linked anti-survivin antisense oligonucleotides (ASON) and CdTe QDs was designed and synthesized. Then, the survivin mRNA down-regulation and the apoptosis of HeLa cells induced by ASON were studied. Systematic experimental results revealed that CdTe-ASON could effectively induce the apoptosis of HeLa cells, while CdTe QDs offered the possibility to visualize the specific intracellular localization of the CdTe-ASON probes strongly associated with their biological functions.

Original language	English
Pages (from-to)	1923-1931
Number of pages	9
Journal	Biomaterials
Volume	32
Issue number	7
DOIs	https://doi.org/10.1016/j.biomaterials.2010.11.024
Publication status	Published - Mar 2011
Externally published	Yes

Keywords

Antisense oligonucleotides
Apoptosis
CdTe QDs
Gene transfection
Intracellular localization

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10.1016/j.biomaterials.2010.11.024

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title = "Quantum dot-antisense oligonucleotide conjugates for multifunctional gene transfection, mRNA regulation, and tracking of biological processes",

abstract = "It was demonstrated that oligonucleotides, independent of their base sequence and length, could effectively induce the cellular uptake of mercapto acid-capped CdTe QDs after the oligonucleotides were covalently attached on the surface of the QDs. Following these experimental observations, a conjugate composed of covalently linked anti-survivin antisense oligonucleotides (ASON) and CdTe QDs was designed and synthesized. Then, the survivin mRNA down-regulation and the apoptosis of HeLa cells induced by ASON were studied. Systematic experimental results revealed that CdTe-ASON could effectively induce the apoptosis of HeLa cells, while CdTe QDs offered the possibility to visualize the specific intracellular localization of the CdTe-ASON probes strongly associated with their biological functions.",

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author = "Yilin Li and Xin Duan and Lihong Jing and Chunhui Yang and Ruirui Qiao and Mingyuan Gao",

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T1 - Quantum dot-antisense oligonucleotide conjugates for multifunctional gene transfection, mRNA regulation, and tracking of biological processes

AU - Li, Yilin

AU - Duan, Xin

AU - Jing, Lihong

AU - Yang, Chunhui

AU - Qiao, Ruirui

AU - Gao, Mingyuan

PY - 2011/3

Y1 - 2011/3

N2 - It was demonstrated that oligonucleotides, independent of their base sequence and length, could effectively induce the cellular uptake of mercapto acid-capped CdTe QDs after the oligonucleotides were covalently attached on the surface of the QDs. Following these experimental observations, a conjugate composed of covalently linked anti-survivin antisense oligonucleotides (ASON) and CdTe QDs was designed and synthesized. Then, the survivin mRNA down-regulation and the apoptosis of HeLa cells induced by ASON were studied. Systematic experimental results revealed that CdTe-ASON could effectively induce the apoptosis of HeLa cells, while CdTe QDs offered the possibility to visualize the specific intracellular localization of the CdTe-ASON probes strongly associated with their biological functions.

AB - It was demonstrated that oligonucleotides, independent of their base sequence and length, could effectively induce the cellular uptake of mercapto acid-capped CdTe QDs after the oligonucleotides were covalently attached on the surface of the QDs. Following these experimental observations, a conjugate composed of covalently linked anti-survivin antisense oligonucleotides (ASON) and CdTe QDs was designed and synthesized. Then, the survivin mRNA down-regulation and the apoptosis of HeLa cells induced by ASON were studied. Systematic experimental results revealed that CdTe-ASON could effectively induce the apoptosis of HeLa cells, while CdTe QDs offered the possibility to visualize the specific intracellular localization of the CdTe-ASON probes strongly associated with their biological functions.

KW - Antisense oligonucleotides

KW - Apoptosis

KW - CdTe QDs

KW - Gene transfection

KW - Intracellular localization

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DO - 10.1016/j.biomaterials.2010.11.024

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SP - 1923

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JO - Biomaterials

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Quantum dot-antisense oligonucleotide conjugates for multifunctional gene transfection, mRNA regulation, and tracking of biological processes

Abstract

Keywords

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