Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain

Laura F Dagley, Carl A White, Yang Liao, Wei Shi, Gordon K Smyth, Jacqueline M Orian, Andrew Emili, Anthony Wayne Purcell

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5 Citations (Scopus)

Abstract

Despite major advances in neuroscience, a comprehensive understanding of the structural and functional components of the adult brain compartments remains to be fully elucidated at a quantitative molecular level. Indeed, over half of the soluble- and membrane-annotated proteins are currently unmapped within online digital brain atlases. In this study, two complementary approaches were used to assess the unique repertoire of proteins enriched within select regions of the adult mouse CNS, including the brain stem, cerebellum, and remaining brain hemispheres. Of the 1200 proteins visualized by 2D-DIGE, approximately 150 (including cytosolic and membrane proteins) were found to exhibit statistically significant changes in relative abundance thus representing putative region-specific brain markers. In addition to using a high-precision (18) O-labeling strategy for the quantitative LC-MS/MS mapping of membrane proteins isolated from myelin-enriched fractions, we have identified over 1000 proteins that have yet to be described in any other mammalian myelin proteome. A comparison of our myelin proteome was made to an existing transcriptome database containing mRNA abundance profiles during oligodendrocyte differentiation and has confirmed statistically significant abundance changes for approximately 500 of these newly mapped proteins, thus revealing new roles in oligodendrocyte and myelin biology. These data offer a resource for the neuroscience community studying the molecular basis for specialized neuronal activities in the CNS and myelin-related disorders. The MS proteomics data associated with this manuscript have been deposited to the ProteomeXchange Consortium with the dataset identifier PXD000327.
Original languageEnglish
Pages (from-to)241 - 261
Number of pages21
JournalProteomics
Volume14
Issue number2-3
DOIs
Publication statusPublished - 2014

Cite this

Dagley, Laura F ; White, Carl A ; Liao, Yang ; Shi, Wei ; Smyth, Gordon K ; Orian, Jacqueline M ; Emili, Andrew ; Purcell, Anthony Wayne. / Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain. In: Proteomics. 2014 ; Vol. 14, No. 2-3. pp. 241 - 261.
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Dagley, LF, White, CA, Liao, Y, Shi, W, Smyth, GK, Orian, JM, Emili, A & Purcell, AW 2014, 'Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain', Proteomics, vol. 14, no. 2-3, pp. 241 - 261. https://doi.org/10.1002/pmic.201300196

Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain. / Dagley, Laura F; White, Carl A; Liao, Yang; Shi, Wei; Smyth, Gordon K; Orian, Jacqueline M; Emili, Andrew; Purcell, Anthony Wayne.

In: Proteomics, Vol. 14, No. 2-3, 2014, p. 241 - 261.

Research output: Contribution to journalArticleResearchpeer-review

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