Abstract Degradation of myelin basic protein (MBP) in human myelin was monitored by electroimmunoblotting. Problems of variation between, as well as within, electroimmunoblots were overcome by the introduction of an internal standard in each sample, thus allowing reproducible quantification of MBP. The Ca2+‐dependent protease acting on MBP was active at endogenous levels of Ca2+ (∼300 μg/g myelin) and was inhibited in the presence of Ca2+ chelators. Extensive degradation of MBP occurred rapidly in the presence of added Ca2+, reaching a plateau after a 1 h incubation (80–85% degradation). The proteolytic activity was not enhanced in the presence of 2‐mercaptoethanol. It was most active at neutral pH and at temperatures approaching physiological conditions. No difference was observed between proteolytic activities of control and multiple sclerotic myelin. It is suggested that fluctuations in the accessibility of free Ca2+ to the protease may lead to the regulation of Ca2+‐activated myelinolysis.
|Number of pages||6|
|Journal||Journal of Neurochemistry|
|Publication status||Published - 1986|
- Calcium‐activated neutral protease
- Multiple sclerosis
- Myelin basic protein
- Quantitative electroimmunoblotting