Quantitation of infectious human immunodeficiency virus using a modified plaque forming assay

R. E. Kiernan, D. A. McPhee, R. R. Doherty

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Abstract

This report describes a modified plaque forming assay which has been used for quantitation of infectious human immunodeficiency virus (HIV) in cell culture supernatant fluid. The number of infectious units determined by the plaque assay correlates closely with the 50% infectious dose determined by a conventional assay in cell culture. In contrast, particle associated reverse transcriptase (RT) activity correlates poorly with the amount of infectious HIV present in culture supernatant fluids.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalJournal of Virological Methods
Volume22
Issue number2-3
DOIs
Publication statusPublished - 1 Jan 1988
Externally publishedYes

Keywords

  • HIV
  • Plaque assay
  • Quantitation

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