Quantitation of green fluorescent protein using a gel-based imaging method

Few Ne Chew, Wen Siang Tan, Tau Chuan Ling, Chon Seng Tan, Beng Ti Tey

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

Green fluorescent protein (GFP) is a versatile reporter protein and has been widely used in biological research. However, its quantitation requires expensive equipment such as a spectrofluorometer. In the current study, a gel documentation imaging system using a native polyacrylamide gel for the quantitation of GFP was developed. The assay was evaluated for its precision, linearity, reproducibility, and sensitivity in the presence of Escherichia coli cells and was compared with the spectrofluorometric method. Using this newly established, gel-based imaging technique; the amount of GFP can be quantified accurately.

Original languageEnglish
Pages (from-to)353-355
Number of pages3
JournalAnalytical Biochemistry
Volume384
Issue number2
DOIs
Publication statusPublished - 15 Jan 2009
Externally publishedYes

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