Quantifying podocyte depletion: theoretical and practical considerations

Victor G. Puelles, John F. Bertram, Marcus J Moeller

Research output: Contribution to journalReview ArticleOtherpeer-review

16 Citations (Scopus)


Podocyte depletion is a central event in the pathogenesis of many glomerular diseases. For this reason, methods to quantify podocyte depletion have become increasingly important. Here, we review currently available methods for quantifying podocyte depletion, including the analysis of glomerular cross-sections, the most important and common stereological methods and newer techniques such as whole glomerular analysis in optically cleared samples. Each method has advantages and limitations. We therefore discuss theoretical and practical considerations to assist the selection of the most appropriate method for an individual study.

Original languageEnglish
Pages (from-to)229-236
Number of pages8
JournalCell and Tissue Research
Issue number1
Publication statusPublished - 1 Jul 2017


  • Glomerulus
  • Kidney disease
  • Microscopy
  • Morphometry
  • Podocyte

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