Quantifying Cellular Internalization with a Fluorescent Click Sensor

Laura I Selby, Luigi Aurelio, Daniel Yuen, Bim Graham, Angus P.R. Johnston

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The ability to determine the amount of material endocytosed by a cell is important for our understanding of cell biology and in the de-sign of effective carriers for drug delivery. To quantify internalization by fluorescence, the signal from material remaining on the cell sur-face must be differentiated from endocytosed material. Sensors for internalization offer advantages over traditional methods for achiev-ing this as they exhibit improved sensitivity, allow for multiple fluorescent markers to be used simultaneously, and are amenable to high throughput analysis. We have developed a small fluorescent internalization sensor, similar in size to a standard fluorescent dye that can be conjugated to proteins and uses the rapid and highly specific bio-orthogonal reaction between a tetrazine and a trans-cyclooctene group to switch off the surface signal. The sensor can be attached to a variety of materials using simple chemistry and is compatible with flow cytometry and fluorescence microscopy, making it a useful tool to study the uptake of material into cells.

Original languageEnglish
Pages (from-to)1182-1189
Number of pages8
JournalACS Sensors
Volume3
Issue number6
DOIs
Publication statusPublished - 2018

Cite this

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Quantifying Cellular Internalization with a Fluorescent Click Sensor. / Selby, Laura I; Aurelio, Luigi; Yuen, Daniel; Graham, Bim; Johnston, Angus P.R.

In: ACS Sensors, Vol. 3, No. 6, 2018, p. 1182-1189.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Selby, Laura I

AU - Aurelio, Luigi

AU - Yuen, Daniel

AU - Graham, Bim

AU - Johnston, Angus P.R.

PY - 2018

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