Quantification of voriconazole in human bronchoalveolar lavage fluid using high-performance liquid chromatography with fluorescence detection

Siow Chin Heng, Roger Leigh Nation, Bronwyn Levvey, Gregory I Snell, Monica Slavin, David Chee Ming Kong

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The quantification of voriconazole concentration in lung epithelial lining fluid to facilitate the management of pulmonary fungal colonisation or aspergillosis is of increasing interest. An accurate and reproducible high-performance liquid chromatography method to quantify voriconazole in human bronchoalveolar lavage (BAL) fluid was developed and validated. BAL samples were concentrated by freeze-drying and reconstituted with water prior to deproteinisation. Separation was achieved with a C18 column employing fluorescence detection (excitation: 260. nm, emission: 370. nm). The calibration curves were linear from 2.5 to 500. ng/mL. The intra- and inter-day precisions were within 7 . Accuracies ranged from 102 to 107 . The clinical applicability was established by successful measurement of voriconazole concentrations in lung transplant recipients. The assay provides an alternative approach for those with negligible access to liquid chromatography-tandem mass spectrometry instrumentation.
Original languageEnglish
Pages (from-to)171 - 175
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Publication statusPublished - 2013

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