TY - JOUR
T1 - Quantification of repertoire diversity of influenza-specific epitopes with predominant public or private TCR usage
AU - Kedzierska, Katherine
AU - Day, E. Bridie
AU - Pi, Jing
AU - Heard, Stephen B.
AU - Doherty, Peter C.
AU - Turner, Stephen J.
AU - Perlman, Stanley
PY - 2006/11/15
Y1 - 2006/11/15
N2 -
The H-2D
b
-restricted CD8 T cell immune response to influenza A is directed at two well-described epitopes, nucleoprotein 366 (NP366) and acid polymerase 224 (PA224). The responses to the two epitopes are very different. The epitope NP366-specific response is dominated by TCR clonotypes that are public (shared by most mice), whereas the epitope PA224-specific response is private (unique within each infected animal). In addition to being public, the NP366-specific response is dominated by a few clonotypes, when T cell clonotypes expressing the Vβ8.3 element are analyzed. Herein, we show that this response is similarly public when the NP366
+
Vβ4
+
CD8 T cell response is analyzed. Furthermore, to determine whether these features resulted in differences in total TCR diversity in the NP366
+
and PA224
+
responses, we quantified the number of different CD8 T clonotypes responding to each epitope. We calculated that 50-550 clonotypes recognized each epitope in individual mice. Thus, although the character of the response to the two epitopes appeared to be different (private and diverse vs public and dominated by a few clonotypes), similar numbers of precursor cells responded to both epitopes and this number was of similar magnitude to that previously reported for other viral CD8 T cell epitopes. Therefore, even in CD8 T cell responses that appear to be oligoclonotypic, the total response is highly diverse.
AB -
The H-2D
b
-restricted CD8 T cell immune response to influenza A is directed at two well-described epitopes, nucleoprotein 366 (NP366) and acid polymerase 224 (PA224). The responses to the two epitopes are very different. The epitope NP366-specific response is dominated by TCR clonotypes that are public (shared by most mice), whereas the epitope PA224-specific response is private (unique within each infected animal). In addition to being public, the NP366-specific response is dominated by a few clonotypes, when T cell clonotypes expressing the Vβ8.3 element are analyzed. Herein, we show that this response is similarly public when the NP366
+
Vβ4
+
CD8 T cell response is analyzed. Furthermore, to determine whether these features resulted in differences in total TCR diversity in the NP366
+
and PA224
+
responses, we quantified the number of different CD8 T clonotypes responding to each epitope. We calculated that 50-550 clonotypes recognized each epitope in individual mice. Thus, although the character of the response to the two epitopes appeared to be different (private and diverse vs public and dominated by a few clonotypes), similar numbers of precursor cells responded to both epitopes and this number was of similar magnitude to that previously reported for other viral CD8 T cell epitopes. Therefore, even in CD8 T cell responses that appear to be oligoclonotypic, the total response is highly diverse.
UR - http://www.scopus.com/inward/record.url?scp=33750839808&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.177.10.6705
DO - 10.4049/jimmunol.177.10.6705
M3 - Article
C2 - 17082583
AN - SCOPUS:33750839808
VL - 177
SP - 6705
EP - 6712
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 10
ER -