Quantification of hepatitis C virus in human liver and serum samples by using LightCycler reverse transcriptase PCR

Peter A. White, Yong Pan, Anthony J. Freeman, George Marinos, Rosemary A. Ffrench, Andrew R. Lloyd, William D. Rawlinson

Research output: Contribution to journalArticleResearchpeer-review

41 Citations (Scopus)

Abstract

A highly sensitive, non-probe-based, real-time quantitative reverse transcriptase PCR was developed for viral load measurements in both serum and liver samples from patients with hepatitis C virus (HCV) infection. With synthetic RNA, the linearity of the approach was conserved over a wide range of HCV copy numbers. There was a strong correlation between hepatic and serum viral load measurements (r = 0.689, P = 0.004, n = 15), indicating that the level of viremia reflected the amount of virus present in the liver.

Original languageEnglish
Pages (from-to)4346-4348
Number of pages3
JournalJournal of Clinical Microbiology
Volume40
Issue number11
DOIs
Publication statusPublished - 1 Nov 2002
Externally publishedYes

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