Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections

Katherine Ververis, Selly P Marzully, Chrishan S Samuel, Tim D Hewitson, Tom C Karagiannis

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)

Abstract

Fluorescent microscope imaging technologies are increasing in their applications and are being used on a wide scale. However methods used to quantify the level of fluorescence intensity are often not utilized-perhaps given the result may be immediately seen, quantification of the data may not seem necessary. However there are a number of reasons given to quantify fluorescent images including the importance of removing potential bias in the data upon observation as well as quantification of large numbers of images gives statistical power to detect subtle changes in experiments. In addition discreet localization of a protein could be detected without selection bias that may not be detectable by eye. Such data will be deemed useful when detecting the levels of HDAC enzymes within cells in order to develop more effective HDAC inhibitor compounds for use against multiple diseased states. Hence, we discuss a methodology devised to analyze fluorescent images using Image J to detect the mean fluorescence intensity of the 11 metal-dependent HDAC enzymes using murine kidney tissue sections as an example.
Original languageEnglish
Title of host publicationKidney Research
Subtitle of host publicationExperimental Protocols
EditorsT D Hewitson, E R Smith, S G Holt
Place of PublicationNew York NY USA
PublisherHumana Press
Pages279-289
Number of pages11
Edition2nd
ISBN (Electronic)978-1-4939-3353-2
ISBN (Print)978-1-4939-3351-8
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Volume1397
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Cite this

Ververis, K., Marzully, S. P., Samuel, C. S., Hewitson, T. D., & Karagiannis, T. C. (2016). Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections. In T. D. Hewitson, E. R. Smith, & S. G. Holt (Eds.), Kidney Research: Experimental Protocols (2nd ed., pp. 279-289). (Methods in Molecular Biology: Springer Protocols; Vol. 1397). Humana Press. https://doi.org/10.1007/978-1-4939-3353-2_19