TY - JOUR
T1 - Qa-1 restricted recognition of foreign antigen by a γδ T-cell hybridoma
AU - Vidović, Damir
AU - Roglić, Mihovil
AU - McKune, Keith
AU - Guerder, Sylvie
AU - MacKay, Charles
AU - Dembić, Zlatko
PY - 1989/1/1
Y1 - 1989/1/1
N2 - DISTINCT T-lymphocyte subsets recognize antigens in conjunction with different classes of major histocompatibility complex (MHC) glycoproteins using the T-cell receptor (TCR), a disulphide-linked heterodimer associated with the CD3 complex on the cell surface1. In general, class I and class II MHC products provide a context for the recognition of foreign antigens by CD8+ and CD4+ T cells, respectively2. This recognition seems to be largely dependent on αβ TCR heterodimers3,4, whereas the function of the second γδ TCR, present on a minor subpopulation of cells, is still unknown5. In the mouse, the existence of six cell-surface MHC class I products (K, D, L, Qa-1, Qa-2 and Tla) has been firmly established by serological, biochemical and genetic evidence6. So far, only the most polymorphic of them, K, D and L ('classical' class I) have been reported as restriction elements for T-cell recognition of foreign antigens1. The function of the relatively invariant Qa and Tla molecules remains unknown6,7. We have made a T-helper cell hybridoma clone (DGT3) that recognizes synthetic copolymer poly(Glu50Tyr50) in the context of Qa-1 cell surface product, and has a CD4-CD8- phenotype. Our studies indicate that DGT3 cells express the γδ TCR on the cell surface, implicating its role in Qa-1-restricted antigen recognition. This is the first evidence that T cells can recognize foreign antigen in association with self Qa product, confirming that Qa molecules not only topologically, but also functionally, belong to the MHC.
AB - DISTINCT T-lymphocyte subsets recognize antigens in conjunction with different classes of major histocompatibility complex (MHC) glycoproteins using the T-cell receptor (TCR), a disulphide-linked heterodimer associated with the CD3 complex on the cell surface1. In general, class I and class II MHC products provide a context for the recognition of foreign antigens by CD8+ and CD4+ T cells, respectively2. This recognition seems to be largely dependent on αβ TCR heterodimers3,4, whereas the function of the second γδ TCR, present on a minor subpopulation of cells, is still unknown5. In the mouse, the existence of six cell-surface MHC class I products (K, D, L, Qa-1, Qa-2 and Tla) has been firmly established by serological, biochemical and genetic evidence6. So far, only the most polymorphic of them, K, D and L ('classical' class I) have been reported as restriction elements for T-cell recognition of foreign antigens1. The function of the relatively invariant Qa and Tla molecules remains unknown6,7. We have made a T-helper cell hybridoma clone (DGT3) that recognizes synthetic copolymer poly(Glu50Tyr50) in the context of Qa-1 cell surface product, and has a CD4-CD8- phenotype. Our studies indicate that DGT3 cells express the γδ TCR on the cell surface, implicating its role in Qa-1-restricted antigen recognition. This is the first evidence that T cells can recognize foreign antigen in association with self Qa product, confirming that Qa molecules not only topologically, but also functionally, belong to the MHC.
UR - http://www.scopus.com/inward/record.url?scp=0024407044&partnerID=8YFLogxK
U2 - 10.1038/340646a0
DO - 10.1038/340646a0
M3 - Article
C2 - 2528072
AN - SCOPUS:0024407044
VL - 340
SP - 646
EP - 650
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6235
ER -