Qa-1 restricted recognition of foreign antigen by a γδ T-cell hybridoma

Damir Vidović, Mihovil Roglić, Keith McKune, Sylvie Guerder, Charles MacKay, Zlatko Dembić

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Abstract

DISTINCT T-lymphocyte subsets recognize antigens in conjunction with different classes of major histocompatibility complex (MHC) glycoproteins using the T-cell receptor (TCR), a disulphide-linked heterodimer associated with the CD3 complex on the cell surface1. In general, class I and class II MHC products provide a context for the recognition of foreign antigens by CD8+ and CD4+ T cells, respectively2. This recognition seems to be largely dependent on αβ TCR heterodimers3,4, whereas the function of the second γδ TCR, present on a minor subpopulation of cells, is still unknown5. In the mouse, the existence of six cell-surface MHC class I products (K, D, L, Qa-1, Qa-2 and Tla) has been firmly established by serological, biochemical and genetic evidence6. So far, only the most polymorphic of them, K, D and L ('classical' class I) have been reported as restriction elements for T-cell recognition of foreign antigens1. The function of the relatively invariant Qa and Tla molecules remains unknown6,7. We have made a T-helper cell hybridoma clone (DGT3) that recognizes synthetic copolymer poly(Glu50Tyr50) in the context of Qa-1 cell surface product, and has a CD4-CD8- phenotype. Our studies indicate that DGT3 cells express the γδ TCR on the cell surface, implicating its role in Qa-1-restricted antigen recognition. This is the first evidence that T cells can recognize foreign antigen in association with self Qa product, confirming that Qa molecules not only topologically, but also functionally, belong to the MHC.

Original languageEnglish
Pages (from-to)646-650
Number of pages5
JournalNature
Volume340
Issue number6235
DOIs
Publication statusPublished - 1 Jan 1989
Externally publishedYes

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