Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS

Hu Tao, Wenjun Liu, Brandi N Simmons, Helen K Harris, Timothy Chilton Cox, Michael A Massiah

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83 Citations (Scopus)

Abstract

We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies. Incubation of inclusion bodies with 10 sarkosyl effectively solubilized >95 of proteins, while high-yield recovery of sarkosyl-solubilized fusion proteins was obtained with a specific ratio of Triton X-100 and CHAPS. We demonstrate for the first time that this combination of three detergents significantly improves binding efficiency of GST and GST fusion proteins to gluthathione (GSH) Sepharose.
Original languageEnglish
Pages (from-to)61 - 64
Number of pages4
JournalBioTechniques
Volume48
Issue number1
DOIs
Publication statusPublished - 2010

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