Purification of transcriptionally active multimeric plasmid DNA using zwitterionic detergent and carbonate apatite non-particles

Recently, we developed a rapid isolation technique for multimeric plasmid based on generation of a protein aggregate using a zwitterionic detergent and alkali. Here we have investigated the roles of different parameters in the whole extraction process to optimise the production of high quality multimeric plasmid DNA. Moreover, we have showed the advantageous effects of nanoparticles to effectively sediment the protein aggregate for smooth elution of multimeric plasmid DNA from it. Finally, quality assessment study has revealed that the isolated multimeric DNA is at least 10 times more transcriptionally active than the monomeric form isolated by the commercially available Qiaget kit