Purification of peptides from solid-phase peptide synthesis with RP-HPLC

Reinhard Boysen; Milton Thomas William Hearn

doi:10.1101/pdb.prot4548

Purification of peptides from solid-phase peptide synthesis with RP-HPLC

Reinhard Boysen
, Milton Thomas William Hearn

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Purification of peptides derived from solid-phase peptide synthesis (SPPS) requires the removal of deletion peptides resulting from incomplete coupling/deprotection steps, from racemization or side-chain rearrangement, and from various chemical substances introduced during the deprotection or cleavage stages of an SPPS procedure. This protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as I?-sheets) that favor supramolecular assembly.

Original language	English
Pages (from-to)	1 - 9
Number of pages	9
Journal	Cold Spring Harbor Protocols
Volume	5
DOIs	https://doi.org/10.1101/pdb.prot4548
Publication status	Published - 2006

Access to Document

10.1101/pdb.prot4548

Cite this

@article{af6d1c1822c34701ba2154cc1e8dd223,

title = "Purification of peptides from solid-phase peptide synthesis with RP-HPLC",

abstract = "Purification of peptides derived from solid-phase peptide synthesis (SPPS) requires the removal of deletion peptides resulting from incomplete coupling/deprotection steps, from racemization or side-chain rearrangement, and from various chemical substances introduced during the deprotection or cleavage stages of an SPPS procedure. This protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as I?-sheets) that favor supramolecular assembly.",

author = "Reinhard Boysen and Hearn, \{Milton Thomas William\}",

year = "2006",

doi = "10.1101/pdb.prot4548",

language = "English",

volume = "5",

pages = "1 -- 9",

journal = "Cold Spring Harbor Protocols",

issn = "1559-6095",

publisher = "Cold Spring Harbor Laboratory Press",

}

TY - JOUR

T1 - Purification of peptides from solid-phase peptide synthesis with RP-HPLC

AU - Boysen, Reinhard

AU - Hearn, Milton Thomas William

PY - 2006

Y1 - 2006

N2 - Purification of peptides derived from solid-phase peptide synthesis (SPPS) requires the removal of deletion peptides resulting from incomplete coupling/deprotection steps, from racemization or side-chain rearrangement, and from various chemical substances introduced during the deprotection or cleavage stages of an SPPS procedure. This protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as I?-sheets) that favor supramolecular assembly.

AB - Purification of peptides derived from solid-phase peptide synthesis (SPPS) requires the removal of deletion peptides resulting from incomplete coupling/deprotection steps, from racemization or side-chain rearrangement, and from various chemical substances introduced during the deprotection or cleavage stages of an SPPS procedure. This protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as I?-sheets) that favor supramolecular assembly.

UR - http://cshprotocols.cshlp.org/content/2006/5/pdb.prot4548.abstract

U2 - 10.1101/pdb.prot4548

DO - 10.1101/pdb.prot4548

M3 - Article

SN - 1559-6095

VL - 5

SP - 1

EP - 9

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

ER -

Purification of peptides from solid-phase peptide synthesis with RP-HPLC

Abstract

Access to Document

Other files and links

Cite this