TY - JOUR
T1 - Purification of peptides from solid-phase peptide synthesis with RP-HPLC
AU - Boysen, Reinhard
AU - Hearn, Milton Thomas William
PY - 2006
Y1 - 2006
N2 - Purification of peptides derived from solid-phase peptide synthesis (SPPS) requires the removal of deletion peptides resulting from incomplete coupling/deprotection steps, from racemization or side-chain rearrangement, and from various chemical substances introduced during the deprotection or cleavage stages of an SPPS procedure. This protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as I?-sheets) that favor supramolecular assembly.
AB - Purification of peptides derived from solid-phase peptide synthesis (SPPS) requires the removal of deletion peptides resulting from incomplete coupling/deprotection steps, from racemization or side-chain rearrangement, and from various chemical substances introduced during the deprotection or cleavage stages of an SPPS procedure. This protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as I?-sheets) that favor supramolecular assembly.
UR - http://cshprotocols.cshlp.org/content/2006/5/pdb.prot4548.abstract
U2 - 10.1101/pdb.prot4548
DO - 10.1101/pdb.prot4548
M3 - Article
VL - 5
SP - 1
EP - 9
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1940-3402
ER -