Purification of peptides from solid-phase peptide synthesis with RP-HPLC

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    Abstract

    Purification of peptides derived from solid-phase peptide synthesis (SPPS) requires the removal of deletion peptides resulting from incomplete coupling/deprotection steps, from racemization or side-chain rearrangement, and from various chemical substances introduced during the deprotection or cleavage stages of an SPPS procedure. This protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as I?-sheets) that favor supramolecular assembly.
    Original languageEnglish
    Pages (from-to)1 - 9
    Number of pages9
    JournalCold Spring Harbor Protocols
    Volume5
    DOIs
    Publication statusPublished - 2006

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