Purification of nucleic acids from gels

Chunfang Zhang, Theodoros Papakonstantinou

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review


    Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are macromolecules composed of single or double strands of nucleotides, with each nucleotide consisting of nitrogenous base (a derivative of purine [adenine and guanine] or pyrimidine [thymine, uracil, and cytosine]) and a sugar (a pentose deosyribose or ribose, together refereed to as nucleoside) as well as a phosphate group. Although the first isolation of DNA was reported by Friedrich Miescher in 1869, it was only in 1953 that the molecular structure of DNA duplex was elucidated by James Watson and Francis Crick. This landmark discovery uncovered the role of DNA as the chmical bearer and transmitter of hereditary features and expedited the development of contemporary molecular biology and biotechnology, with its full impact yet to be felt in the years to come.
    Original languageEnglish
    Title of host publicationHandbook of Nucleic Acid Purification
    EditorsDongyou Liu
    Place of PublicationBoca Raton Florida USA
    PublisherCRC Press
    Pages537 - 555
    Number of pages19
    ISBN (Print)978-1-4200-7096-5
    Publication statusPublished - 2009

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