Purification, crystallization and preliminary X-ray crystallographic analysis of the putative Vibrio parahaemolyticus resuscitation-promoting factor YeaZ

Inci Aydin, Alexandra Dimitropoulos, Shih Hsun Chen, Connor Thomas, Anna Roujeinikova

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Vibrio parahaemolyticus is a human pathogen associated with gastroenteritis caused by the ingestion of contaminated raw seafood. V. parahaemolyticus is able to survive exposure to low temperatures typical of those used for the refrigeration of foods by entering a viable but nonculturable (VBNC) state. The VBNC cells can regain culturability and renewed ability to cause infection upon temperature upshift. The resuscitation-promoting factor protein (Rpf, YeaZ) plays a key role in reactivation of growth. Crystals of V. parahaemolyticus YeaZ have been grown using the hanging-drop vapour-diffusion method with polyethylene glycol as a precipitating agent. The crystals belonged to the primitive monoclinic space group P21, with unit-cell parameters a = 81.7, b = 63.8, c = 82.3 Å, β = 105° and four subunits in the asymmetric unit. A complete X-ray diffraction data set was collected from a single crystal to 3.1 Å resolution.
Original languageEnglish
Pages (from-to)604-607
Number of pages4
JournalActa Crystallographica. Section F: Structural Biology Communications
VolumeF67
Issue numberPart 5
DOIs
Publication statusPublished - 2011

Keywords

  • YeaZ
  • resuscitation-promoting factors
  • vibrio parahaemolyticus

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