Purification, crystallization and preliminary crystallographic analysis of banyan peroxidase

Anurag Sharma, Gottfried J. Palm, Moni Kumari, Santosh Panjikar, M. V. Jagannadham, Winfried Hinrichs

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1 Citation (Scopus)


Plant peroxidases are extensively used in a wide range of biotechnological applications owing to their high environmental and thermal stability. A new peroxidase, named banyan peroxidase, was purified from the latex of Ficus benghalensis and crystallized. X-ray diffraction data were collected from native crystals and from bromide and xenon derivatives to resolutions of up to 1.66 Å in the trigonal space group P3221, with unit-cell parameters a = b = 73.1, c = 164.6 Å. The anomalous signal of the intrinsic iron and calcium ions was sufficient for structure solution by SAD, although the sequence is not yet known.

Original languageEnglish
Pages (from-to)931-934
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Issue number8
Publication statusPublished - Aug 2012
Externally publishedYes


  • Ficus benghalensis
  • latex
  • plant peroxidases

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