Purification, crystallization and preliminary crystallographic analysis of biotin protein ligase from Staphylococcus aureus

Nicole Renee Pendini, Steven W Polyak, Grant William Booker, John Campbell Wallace, Matthew Charles James Wilce

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10 Citations (Scopus)

Abstract

Biotin protein ligase from Staphylococcus aureus catalyses the biotinylation of acetyl-CoA carboxylase and pyruvate carboxylase. Recombinant biotin protein ligase from S. aureus has been cloned, expressed and purified. Crystals were grown using the hanging-drop vapour-diffusion method using PEG 8000 as the precipitant at 295 K. X-ray diffraction data were collected to 2.3 A resolution from crystals using synchrotron X-ray radiation at 100 K. The diffraction was consistent with the tetragonal space group P4(2)2(1)2, with unit-cell parameters a = b = 93.665, c = 131.95.
Original languageEnglish
Pages (from-to)520 - 523
Number of pages4
Journal Acta Crystallographica Section F: Structural Biology Communications
Volume64
Issue numberPt 6
DOIs
Publication statusPublished - 2008

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