Purification and characterization of pectin acetylesterase from orange peel

Gary Williamson

doi:10.1016/0031-9422(91)83701-L

Purification and characterization of pectin acetylesterase from orange peel

Gary Williamson

Research output: Contribution to journal › Article › Research › peer-review

43 Citations (Scopus)

Abstract

Pectin acetylesterase was purified from orange peel using ammonium sulphate fractionation, followed by chromatography on Sephadex G-25 superfine, mono S and Superose 6. The purified enzyme has a M_r of 29 000. It has no pectin methylesterase activity, but rapidly de-esterifies triacetin. The pectin acetylesterase activity is increased 4.8-fold by prior incubation of sugarbeet pectin with pectin methylesterase, which suggests that the acetylesterase preferentially deacetylates galacturonyl residues containing a free C-6 carboxy group.

Original language	English
Pages (from-to)	445-449
Number of pages	5
Journal	Phytochemistry
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/0031-9422(91)83701-L
Publication status	Published - 2 Jan 1991
Externally published	Yes

Keywords

acetylesterase
Citrus sinensis
enzyme purification
orange peel
pectin
pectin acetylesterase
pectin methylesterase
pectinesterase.
Rutaceae

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10.1016/0031-9422(91)83701-L

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title = "Purification and characterization of pectin acetylesterase from orange peel",

abstract = "Pectin acetylesterase was purified from orange peel using ammonium sulphate fractionation, followed by chromatography on Sephadex G-25 superfine, mono S and Superose 6. The purified enzyme has a Mr of 29 000. It has no pectin methylesterase activity, but rapidly de-esterifies triacetin. The pectin acetylesterase activity is increased 4.8-fold by prior incubation of sugarbeet pectin with pectin methylesterase, which suggests that the acetylesterase preferentially deacetylates galacturonyl residues containing a free C-6 carboxy group.",

keywords = "acetylesterase, Citrus sinensis, enzyme purification, orange peel, pectin, pectin acetylesterase, pectin methylesterase, pectinesterase., Rutaceae",

author = "Gary Williamson",

year = "1991",

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doi = "10.1016/0031-9422(91)83701-L",

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AU - Williamson, Gary

PY - 1991/1/2

Y1 - 1991/1/2

N2 - Pectin acetylesterase was purified from orange peel using ammonium sulphate fractionation, followed by chromatography on Sephadex G-25 superfine, mono S and Superose 6. The purified enzyme has a Mr of 29 000. It has no pectin methylesterase activity, but rapidly de-esterifies triacetin. The pectin acetylesterase activity is increased 4.8-fold by prior incubation of sugarbeet pectin with pectin methylesterase, which suggests that the acetylesterase preferentially deacetylates galacturonyl residues containing a free C-6 carboxy group.

AB - Pectin acetylesterase was purified from orange peel using ammonium sulphate fractionation, followed by chromatography on Sephadex G-25 superfine, mono S and Superose 6. The purified enzyme has a Mr of 29 000. It has no pectin methylesterase activity, but rapidly de-esterifies triacetin. The pectin acetylesterase activity is increased 4.8-fold by prior incubation of sugarbeet pectin with pectin methylesterase, which suggests that the acetylesterase preferentially deacetylates galacturonyl residues containing a free C-6 carboxy group.

KW - acetylesterase

KW - Citrus sinensis

KW - enzyme purification

KW - orange peel

KW - pectin

KW - pectin acetylesterase

KW - pectin methylesterase

KW - pectinesterase.

KW - Rutaceae

UR - https://www.scopus.com/pages/publications/0000849712

U2 - 10.1016/0031-9422(91)83701-L

DO - 10.1016/0031-9422(91)83701-L

M3 - Article

AN - SCOPUS:0000849712

SN - 0031-9422

VL - 30

SP - 445

EP - 449

JO - Phytochemistry

JF - Phytochemistry

IS - 2

ER -

Purification and characterization of pectin acetylesterase from orange peel

Abstract

Keywords

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