Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa

Raymond S. Norton, Gabriele Bobek, John O. Ivanov, Murray Thomson, Eva Fiala-Beer, Robert L. Moritz, Richard J. Simpson

Research output: Contribution to journalArticleResearchpeer-review

39 Citations (Scopus)

Abstract

R. S. Norton, G. Bobek, J. O. Ivanov, M. Thomson, E. Fiala-Beer, R. L. Moritz and R. J. Simpson. Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa. Toxicon 28, 29-41, 1990.-Three new proteins with cardiac stimulatory and haemolytic activity, designated tenebrosins-A, -B and -C, have been purified from the Australian sea anemone Actinia tenebrosa. These proteins are basic (pI ≥ 9.4), have mol. wt of about 20,000, and have very similar amino acid compositions and N-terminal amino acid sequences. None of the proteins contains cysteine or cystine residues. On isolated, spontaneously beating guinea pig atria they exhibit at 1-2 nM strong positive inotropic and slight to moderate chronotropic effects. In some cases a transient negative inotropic effect occurs prior to onset of the positive inotropic response. The proteins are also haemolytic, producing 50% haemolysis of guinea pig erythrocytes at concentrations similar to those showing positive inotropic effects.

Original languageEnglish
Pages (from-to)29-41
Number of pages13
JournalToxicon
Volume28
Issue number1
DOIs
Publication statusPublished - 1990
Externally publishedYes

Cite this

Norton, Raymond S. ; Bobek, Gabriele ; Ivanov, John O. ; Thomson, Murray ; Fiala-Beer, Eva ; Moritz, Robert L. ; Simpson, Richard J. / Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa. In: Toxicon. 1990 ; Vol. 28, No. 1. pp. 29-41.
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Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa. / Norton, Raymond S.; Bobek, Gabriele; Ivanov, John O.; Thomson, Murray; Fiala-Beer, Eva; Moritz, Robert L.; Simpson, Richard J.

In: Toxicon, Vol. 28, No. 1, 1990, p. 29-41.

Research output: Contribution to journalArticleResearchpeer-review

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