Abstract
R. S. Norton, G. Bobek, J. O. Ivanov, M. Thomson, E. Fiala-Beer, R. L. Moritz and R. J. Simpson. Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa. Toxicon 28, 29-41, 1990.-Three new proteins with cardiac stimulatory and haemolytic activity, designated tenebrosins-A, -B and -C, have been purified from the Australian sea anemone Actinia tenebrosa. These proteins are basic (pI ≥ 9.4), have mol. wt of about 20,000, and have very similar amino acid compositions and N-terminal amino acid sequences. None of the proteins contains cysteine or cystine residues. On isolated, spontaneously beating guinea pig atria they exhibit at 1-2 nM strong positive inotropic and slight to moderate chronotropic effects. In some cases a transient negative inotropic effect occurs prior to onset of the positive inotropic response. The proteins are also haemolytic, producing 50% haemolysis of guinea pig erythrocytes at concentrations similar to those showing positive inotropic effects.
| Original language | English |
|---|---|
| Pages (from-to) | 29-41 |
| Number of pages | 13 |
| Journal | Toxicon |
| Volume | 28 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1990 |
| Externally published | Yes |
Cite this
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Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa. / Norton, Raymond S.; Bobek, Gabriele; Ivanov, John O.; Thomson, Murray; Fiala-Beer, Eva; Moritz, Robert L.; Simpson, Richard J.
In: Toxicon, Vol. 28, No. 1, 1990, p. 29-41.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa
AU - Norton, Raymond S.
AU - Bobek, Gabriele
AU - Ivanov, John O.
AU - Thomson, Murray
AU - Fiala-Beer, Eva
AU - Moritz, Robert L.
AU - Simpson, Richard J.
PY - 1990
Y1 - 1990
N2 - R. S. Norton, G. Bobek, J. O. Ivanov, M. Thomson, E. Fiala-Beer, R. L. Moritz and R. J. Simpson. Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa. Toxicon 28, 29-41, 1990.-Three new proteins with cardiac stimulatory and haemolytic activity, designated tenebrosins-A, -B and -C, have been purified from the Australian sea anemone Actinia tenebrosa. These proteins are basic (pI ≥ 9.4), have mol. wt of about 20,000, and have very similar amino acid compositions and N-terminal amino acid sequences. None of the proteins contains cysteine or cystine residues. On isolated, spontaneously beating guinea pig atria they exhibit at 1-2 nM strong positive inotropic and slight to moderate chronotropic effects. In some cases a transient negative inotropic effect occurs prior to onset of the positive inotropic response. The proteins are also haemolytic, producing 50% haemolysis of guinea pig erythrocytes at concentrations similar to those showing positive inotropic effects.
AB - R. S. Norton, G. Bobek, J. O. Ivanov, M. Thomson, E. Fiala-Beer, R. L. Moritz and R. J. Simpson. Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa. Toxicon 28, 29-41, 1990.-Three new proteins with cardiac stimulatory and haemolytic activity, designated tenebrosins-A, -B and -C, have been purified from the Australian sea anemone Actinia tenebrosa. These proteins are basic (pI ≥ 9.4), have mol. wt of about 20,000, and have very similar amino acid compositions and N-terminal amino acid sequences. None of the proteins contains cysteine or cystine residues. On isolated, spontaneously beating guinea pig atria they exhibit at 1-2 nM strong positive inotropic and slight to moderate chronotropic effects. In some cases a transient negative inotropic effect occurs prior to onset of the positive inotropic response. The proteins are also haemolytic, producing 50% haemolysis of guinea pig erythrocytes at concentrations similar to those showing positive inotropic effects.
UR - http://www.scopus.com/inward/record.url?scp=0025332205&partnerID=8YFLogxK
U2 - 10.1016/0041-0101(90)90004-Q
DO - 10.1016/0041-0101(90)90004-Q
M3 - Article
C2 - 1970442
AN - SCOPUS:0025332205
VL - 28
SP - 29
EP - 41
JO - Toxicon : official journal of the International Society on Toxinology
JF - Toxicon : official journal of the International Society on Toxinology
SN - 0041-0101
IS - 1
ER -