TY - JOUR
T1 - Purification and assay of bovine parathyroid hormone by reversed-phase high-performance liquid chromatography
AU - Zanelli, Joan M.
AU - O'Hare, M. J.
AU - Nice, E. C.
AU - Corran, P. H.
PY - 1981/4/10
Y1 - 1981/4/10
N2 - Reversed-phase high-performance liquid chromatography (HPLC) has been used to purify a crude extract of bovine parathyroid glands, in a single run on an analytical column, to give a high yield of homogeneous material with full bioactivity in in vivo bioassay. Bovine parathyroid hormone (bPTH) prepared and purified by conventional procedures has been rapidly and quantitatively separated from its oxidation and other degradation products, from hormone fragments and from non-hormonal contaminants. Recovery of bPTH, monitored by region-specific immunoassays, in vivo bioassay and re-chromatography on HPLC was > 93%. The detection limit of the HPLC system, using endogenous tryptophan fluorescence, was 20 ng bPTH.
AB - Reversed-phase high-performance liquid chromatography (HPLC) has been used to purify a crude extract of bovine parathyroid glands, in a single run on an analytical column, to give a high yield of homogeneous material with full bioactivity in in vivo bioassay. Bovine parathyroid hormone (bPTH) prepared and purified by conventional procedures has been rapidly and quantitatively separated from its oxidation and other degradation products, from hormone fragments and from non-hormonal contaminants. Recovery of bPTH, monitored by region-specific immunoassays, in vivo bioassay and re-chromatography on HPLC was > 93%. The detection limit of the HPLC system, using endogenous tryptophan fluorescence, was 20 ng bPTH.
UR - http://www.scopus.com/inward/record.url?scp=0019829683&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(00)80068-7
DO - 10.1016/S0378-4347(00)80068-7
M3 - Article
C2 - 7251777
AN - SCOPUS:0019829683
SN - 1387-2273
VL - 223
SP - 59
EP - 67
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -