Purification and assay of bovine parathyroid hormone by reversed-phase high-performance liquid chromatography

Joan M. Zanelli, M. J. O'Hare, E. C. Nice, P. H. Corran

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

Reversed-phase high-performance liquid chromatography (HPLC) has been used to purify a crude extract of bovine parathyroid glands, in a single run on an analytical column, to give a high yield of homogeneous material with full bioactivity in in vivo bioassay. Bovine parathyroid hormone (bPTH) prepared and purified by conventional procedures has been rapidly and quantitatively separated from its oxidation and other degradation products, from hormone fragments and from non-hormonal contaminants. Recovery of bPTH, monitored by region-specific immunoassays, in vivo bioassay and re-chromatography on HPLC was > 93%. The detection limit of the HPLC system, using endogenous tryptophan fluorescence, was 20 ng bPTH.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume223
Issue number1
DOIs
Publication statusPublished - 10 Apr 1981
Externally publishedYes

Cite this