Abstract
| Original language | English |
|---|---|
| Pages (from-to) | L232 - L241 |
| Number of pages | 10 |
| Journal | American Journal of Physiology - Lung Cellular and Molecular Physiology |
| Volume | 299 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2010 |
Cite this
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Pulmonary vascular and alveolar development in preterm lambs chronically colonized with Ureaplasma parvum. / Polglase, Graeme; Dalton, Richard; Nitsos, Ilias; Knox, Christine; Pillow, Jane; Jobe, Alan; Moss, Timothy; Newnham, John; Kallapur, Suhas.
In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 299, No. 2, 2010, p. L232 - L241.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Pulmonary vascular and alveolar development in preterm lambs chronically colonized with Ureaplasma parvum
AU - Polglase, Graeme
AU - Dalton, Richard
AU - Nitsos, Ilias
AU - Knox, Christine
AU - Pillow, Jane
AU - Jobe, Alan
AU - Moss, Timothy
AU - Newnham, John
AU - Kallapur, Suhas
PY - 2010
Y1 - 2010
N2 - Ureaplasma species, the most commonly isolated microorganisms in women with chorioamnionitis, are associated with preterm delivery. Chorioamnionitis increases the risk and severity of bronchopulmonary dysplasia and persistent pulmonary hypertension in newborns. It is not known whether the timing of exposure to inflammation in utero is an important contributor to the pathogenesis of bronchopulmonary dysplasia. We hypothesized that chronic inflammation would alter the pulmonary air space and vascular development after 70 days of exposure to infection. Pregnant ewes were given intra-amniotic injection of Ureaplasma parvum serovars 3 or 6 at low (2 x 10(4) cfu) or high doses (2 x 10(7) cfu) or media (controls) at 55 days gestational age. Fetuses were delivered at 125 days (term = 150 days). U. parvum was grown from the lungs of all exposed fetuses, and neutrophils and monocytes were increased in the air spaces. Lung mRNA expression of IL-1beta and IL-8, but not IL-6, was modestly increased in U. parvum-exposed fetuses. U. parvum exposure increased surfactant and improved lung gas volumes. The changes in lung inflammation and maturation were independent of serovar or dose. Exposure to U. parvum did not change multiple indices of air space or vascular development. Parenchymal elastin and collagen content were similar between groups. Expression of several endothelial proteins and pulmonary resistance arteriolar media thickness were also not different between groups. We conclude that chronic exposure to U. parvum does not cause sustained effects on air space or vascular development in premature lambs.
AB - Ureaplasma species, the most commonly isolated microorganisms in women with chorioamnionitis, are associated with preterm delivery. Chorioamnionitis increases the risk and severity of bronchopulmonary dysplasia and persistent pulmonary hypertension in newborns. It is not known whether the timing of exposure to inflammation in utero is an important contributor to the pathogenesis of bronchopulmonary dysplasia. We hypothesized that chronic inflammation would alter the pulmonary air space and vascular development after 70 days of exposure to infection. Pregnant ewes were given intra-amniotic injection of Ureaplasma parvum serovars 3 or 6 at low (2 x 10(4) cfu) or high doses (2 x 10(7) cfu) or media (controls) at 55 days gestational age. Fetuses were delivered at 125 days (term = 150 days). U. parvum was grown from the lungs of all exposed fetuses, and neutrophils and monocytes were increased in the air spaces. Lung mRNA expression of IL-1beta and IL-8, but not IL-6, was modestly increased in U. parvum-exposed fetuses. U. parvum exposure increased surfactant and improved lung gas volumes. The changes in lung inflammation and maturation were independent of serovar or dose. Exposure to U. parvum did not change multiple indices of air space or vascular development. Parenchymal elastin and collagen content were similar between groups. Expression of several endothelial proteins and pulmonary resistance arteriolar media thickness were also not different between groups. We conclude that chronic exposure to U. parvum does not cause sustained effects on air space or vascular development in premature lambs.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=20495079
U2 - 10.1152/ajplung.00369.2009
DO - 10.1152/ajplung.00369.2009
M3 - Article
VL - 299
SP - L232 - L241
JO - American Journal of Physiology - Lung Cellular and Molecular Physiology
JF - American Journal of Physiology - Lung Cellular and Molecular Physiology
SN - 1040-0605
IS - 2
ER -