Pseudomonas infection responsive liquid crystals for glycoside hydrolase and antibiotic combination

Chelsea R. Thorn, Clive A. Prestidge, Ben J. Boyd, Nicky Thomas

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)

Abstract

Bacterial biofilms account for up to 80% of all communityacquired infections for which bacterial eradication is currently not achievable using conventional antimicrobial treatments. The protective matrix that engulfs biofilm-associated bacteria frequently renders antibiotics ineffective. Glycoside hydrolases are a class of enzymes that break down the biofilm matrix, thereby increasing the effectiveness of antibiotics. Herein, nanostructured liquid crystals composed of glyceryl monooleate (GMO) were investigated as an infection responsive delivery system for alginate lyase (glycoside hydrolase) and gentamicin (antibiotic) to treat Pseudomonas biofilms. The presence of Pseudomonas lipase triggered the release of alginate lyase and gentamicin from the GMO liquid crystals. Treatment with the liquid crystals containing alginate lyase and gentamicin resulted in a greater than 2-log reduction in mucoid Pseudomonas aeruginosa (clinical isolate) biofilm. The anti-biofilm activity of alginate lyase and gentamicin from the liquid crystals was sustained for 2 days and equivalent to the respective unformulated solution treatments. Accordingly, GMO based liquid crystals are a promising responsive delivery system for alginate lyase and gentamicin to combat topical Pseudomonas infections.

Original languageEnglish
Pages (from-to)281-288
Number of pages8
JournalACS Applied Bio Materials
Volume1
Issue number2
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Alginate lyase
  • Biofilm
  • Gentamicin
  • Glyceryl monooleate
  • Liquid crystals

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