Pseudomonas aeruginosa utilises host‐derived polyamines to facilitate antimicrobial tolerance

Chowdhury Mehedi Hasan, Angharad E. Green, Adrienne A. Cox, Jack White, Trevor Jones, Craig Winstanley, Aras Kadioglu, Megan Wright, Daniel R. Neill, Joanne L. Fothergill

Research output: Other contributionResearch

Abstract

Pseudomonas aeruginosa undergoes diversification during infection of the cystic fibrosis (CF) lung. Understanding these changes requires model systems that capture the complexity of the CF lung environment. We previously identified loss-of-function mutations in the two-component regulatory system sensor kinase gene pmrB, in P. aeruginosa from CF and from experimental infection of mice. Here, we demonstrate that whilst such mutations lower in vitro MICs for multiple antimicrobial classes, this is not reflected in increased antibiotic susceptibility in vivo. Loss of PmrB impairs aminoarabinose modification of lipopolysaccharide, increasing the negative charge of the outer membrane and promoting uptake of cationic antimicrobials. However, in vivo, this can be offset by increased membrane binding of other positively charged molecules present in lungs. The polyamine spermidine readily coats the surface of PmrB-deficient P. aeruginosa, reducing susceptibility to antibiotics that rely on charge differences to bind the outer membrane and increasing biofilm formation. Spermidine is elevated in lungs during P. aeruginosa infection in mice and during episodes of antimicrobial treatment in people with CF. These findings highlight the need to study antimicrobial resistance under clinically relevant environmental conditions. Microbial mutations carrying fitness costs in vitro may be advantageous during infection, where host resources can be utilised.
Original languageEnglish
Typepreprint
Media of outputbioRxiv
PublisherbioRxiv
Number of pages26
DOIs
Publication statusPublished - 26 Jan 2022
Externally publishedYes

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