Protocols to study aging in Drosophila

Matthew D W Piper; Linda Partridge

doi:10.1007/978-1-4939-6371-3_18

Protocols to study aging in Drosophila

Matthew D W Piper
, Linda Partridge

School of Biological Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

78 Citations (Scopus)

Abstract

The fruit fly Drosophila melanogaster offers a host of advantages for studying the biology of aging: a wellunderstood biology, a wide range of genetic reagents, well-defined dietary requirements, and a relatively short life span, with a median of ~80 days and maximum ~100 days. Several phenotypes can be used to assess the aging process, but the simplest and most widely used metric is length of life. Here we describe a standard life span assay for Drosophila housed on a simple sugar/yeast diet.

Original language	English
Title of host publication	Drosophila: Methods and Protocols
Editors	Christian Dahmann
Place of Publication	New York NY USA
Publisher	Humana Press
Pages	291-302
Number of pages	12
Edition	2nd
ISBN (Electronic)	9781493963713
ISBN (Print)	9781493963690
DOIs	https://doi.org/10.1007/978-1-4939-6371-3_18
Publication status	Published - 12 Oct 2016

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	1478
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Aging
Backcross
Diet
Drosophila melanogaster
Genetic interventions
Life span
Method
Pharmacological interventions

Access to Document

10.1007/978-1-4939-6371-3_18

Cite this

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T1 - Protocols to study aging in Drosophila

AU - Piper, Matthew D W

AU - Partridge, Linda

PY - 2016/10/12

Y1 - 2016/10/12

N2 - The fruit fly Drosophila melanogaster offers a host of advantages for studying the biology of aging: a wellunderstood biology, a wide range of genetic reagents, well-defined dietary requirements, and a relatively short life span, with a median of ~80 days and maximum ~100 days. Several phenotypes can be used to assess the aging process, but the simplest and most widely used metric is length of life. Here we describe a standard life span assay for Drosophila housed on a simple sugar/yeast diet.

AB - The fruit fly Drosophila melanogaster offers a host of advantages for studying the biology of aging: a wellunderstood biology, a wide range of genetic reagents, well-defined dietary requirements, and a relatively short life span, with a median of ~80 days and maximum ~100 days. Several phenotypes can be used to assess the aging process, but the simplest and most widely used metric is length of life. Here we describe a standard life span assay for Drosophila housed on a simple sugar/yeast diet.

KW - Aging

KW - Backcross

KW - Diet

KW - Drosophila melanogaster

KW - Genetic interventions

KW - Life span

KW - Method

KW - Pharmacological interventions

UR - https://www.scopus.com/pages/publications/84991453645

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DO - 10.1007/978-1-4939-6371-3_18

M3 - Chapter (Book)

AN - SCOPUS:84991453645

SN - 9781493963690

T3 - Methods in Molecular Biology

SP - 291

EP - 302

BT - Drosophila: Methods and Protocols

A2 - Dahmann, Christian

PB - Humana Press

CY - New York NY USA

ER -

Protocols to study aging in Drosophila

Abstract

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Keywords

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Understanding diet designs that break life history trade-offs

Cite this

Protocols to study aging in Drosophila

Abstract

Publication series

Keywords

Access to Document

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Projects

Understanding diet designs that break life history trade-offs

Cite this