Protocols to study aging in Drosophila

Matthew D W Piper; Linda Partridge

doi:10.1007/978-1-4939-6371-3_18

Protocols to study aging in Drosophila

Matthew D W Piper, Linda Partridge

School of Biological Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

27 Citations (Scopus)

Abstract

The fruit fly Drosophila melanogaster offers a host of advantages for studying the biology of aging: a wellunderstood biology, a wide range of genetic reagents, well-defined dietary requirements, and a relatively short life span, with a median of ~80 days and maximum ~100 days. Several phenotypes can be used to assess the aging process, but the simplest and most widely used metric is length of life. Here we describe a standard life span assay for Drosophila housed on a simple sugar/yeast diet.

Original language	English
Title of host publication	Drosophila: Methods and Protocols
Editors	Christian Dahmann
Place of Publication	New York NY USA
Publisher	Humana Press
Pages	291-302
Number of pages	12
Edition	2
ISBN (Electronic)	9781493963713
ISBN (Print)	978149396369
DOIs	https://doi.org/10.1007/978-1-4939-6371-3_18
Publication status	Published - 12 Oct 2016

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	1478
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Aging
Backcross
Diet
Drosophila melanogaster
Genetic interventions
Life span
Method
Pharmacological interventions

Access to Document

10.1007/978-1-4939-6371-3_18

Cite this

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AU - Partridge, Linda

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AB - The fruit fly Drosophila melanogaster offers a host of advantages for studying the biology of aging: a wellunderstood biology, a wide range of genetic reagents, well-defined dietary requirements, and a relatively short life span, with a median of ~80 days and maximum ~100 days. Several phenotypes can be used to assess the aging process, but the simplest and most widely used metric is length of life. Here we describe a standard life span assay for Drosophila housed on a simple sugar/yeast diet.

KW - Aging

KW - Backcross

KW - Diet

KW - Drosophila melanogaster

KW - Genetic interventions

KW - Life span

KW - Method

KW - Pharmacological interventions

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T3 - Methods in Molecular Biology

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EP - 302

BT - Drosophila: Methods and Protocols

A2 - Dahmann, Christian

PB - Humana Press

CY - New York NY USA

ER -

Protocols to study aging in Drosophila

Abstract

Publication series

Keywords

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Understanding diet designs that break life history trade-offs

Cite this

Protocols to study aging in Drosophila

Abstract

Publication series

Keywords

Access to Document

Other files and links

Projects

Understanding diet designs that break life history trade-offs

Cite this