Proteomic characterization of midproliferative and midsecretory human endometrium

Jenny I C Chen, Natalie J. Hannan, Yunxian Mak, Peter K. Nicholls, Jin Zhang, Adam Rainczuk, Peter G. Stanton, David M. Robertson, Lois A. Salamonsen, Andrew N. Stephens

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIa, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.

Original languageEnglish
Pages (from-to)2032-2044
Number of pages13
JournalJournal of Proteome Research
Volume8
Issue number4
DOIs
Publication statusPublished - 3 Apr 2009
Externally publishedYes

Keywords

  • 2D PAGE
  • CLIC1
  • Endometrium
  • Mass spectrometry
  • PGRMC1
  • Proliferative and secretory phases of the menstrual cycle
  • Proteome
  • Rho-GDIα
  • Two-dimensional differential in-gel electrophoresis (2D DIGE)

Cite this

Chen, J. I. C., Hannan, N. J., Mak, Y., Nicholls, P. K., Zhang, J., Rainczuk, A., ... Stephens, A. N. (2009). Proteomic characterization of midproliferative and midsecretory human endometrium. Journal of Proteome Research, 8(4), 2032-2044. https://doi.org/10.1021/pr801024g
Chen, Jenny I C ; Hannan, Natalie J. ; Mak, Yunxian ; Nicholls, Peter K. ; Zhang, Jin ; Rainczuk, Adam ; Stanton, Peter G. ; Robertson, David M. ; Salamonsen, Lois A. ; Stephens, Andrew N. / Proteomic characterization of midproliferative and midsecretory human endometrium. In: Journal of Proteome Research. 2009 ; Vol. 8, No. 4. pp. 2032-2044.
@article{fa9e4d3700a9480988fe51f348093542,
title = "Proteomic characterization of midproliferative and midsecretory human endometrium",
abstract = "This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIa, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.",
keywords = "2D PAGE, CLIC1, Endometrium, Mass spectrometry, PGRMC1, Proliferative and secretory phases of the menstrual cycle, Proteome, Rho-GDIα, Two-dimensional differential in-gel electrophoresis (2D DIGE)",
author = "Chen, {Jenny I C} and Hannan, {Natalie J.} and Yunxian Mak and Nicholls, {Peter K.} and Jin Zhang and Adam Rainczuk and Stanton, {Peter G.} and Robertson, {David M.} and Salamonsen, {Lois A.} and Stephens, {Andrew N.}",
year = "2009",
month = "4",
day = "3",
doi = "10.1021/pr801024g",
language = "English",
volume = "8",
pages = "2032--2044",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "4",

}

Proteomic characterization of midproliferative and midsecretory human endometrium. / Chen, Jenny I C; Hannan, Natalie J.; Mak, Yunxian; Nicholls, Peter K.; Zhang, Jin; Rainczuk, Adam; Stanton, Peter G.; Robertson, David M.; Salamonsen, Lois A.; Stephens, Andrew N.

In: Journal of Proteome Research, Vol. 8, No. 4, 03.04.2009, p. 2032-2044.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Proteomic characterization of midproliferative and midsecretory human endometrium

AU - Chen, Jenny I C

AU - Hannan, Natalie J.

AU - Mak, Yunxian

AU - Nicholls, Peter K.

AU - Zhang, Jin

AU - Rainczuk, Adam

AU - Stanton, Peter G.

AU - Robertson, David M.

AU - Salamonsen, Lois A.

AU - Stephens, Andrew N.

PY - 2009/4/3

Y1 - 2009/4/3

N2 - This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIa, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.

AB - This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIa, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.

KW - 2D PAGE

KW - CLIC1

KW - Endometrium

KW - Mass spectrometry

KW - PGRMC1

KW - Proliferative and secretory phases of the menstrual cycle

KW - Proteome

KW - Rho-GDIα

KW - Two-dimensional differential in-gel electrophoresis (2D DIGE)

UR - http://www.scopus.com/inward/record.url?scp=65249134393&partnerID=8YFLogxK

U2 - 10.1021/pr801024g

DO - 10.1021/pr801024g

M3 - Article

VL - 8

SP - 2032

EP - 2044

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 4

ER -

Chen JIC, Hannan NJ, Mak Y, Nicholls PK, Zhang J, Rainczuk A et al. Proteomic characterization of midproliferative and midsecretory human endometrium. Journal of Proteome Research. 2009 Apr 3;8(4):2032-2044. https://doi.org/10.1021/pr801024g