Proteomic characterization of midproliferative and midsecretory human endometrium

Jenny I C Chen, Natalie J. Hannan, Yunxian Mak, Peter K. Nicholls, Jin Zhang, Adam Rainczuk, Peter G. Stanton, David M. Robertson, Lois A. Salamonsen, Andrew N. Stephens

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Abstract

This study aimed to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles by 2D differential in-gel electrophoresis (DIGE). A total of 196 out of 1017 spots were differentially expressed (p < 0.05). Mass spectrometry identified 76 proteins representing 41 different gene products. Immunohistochemistry confirmed the observed changes in 3 representative proteins (Rho-GDIa, CLIC1, PGRMC1). Biological pathway analysis identified the Jnk and EGF signaling pathways as key regulators of protein expression in the midsecretory phase of endometrial proteome.

Original languageEnglish
Pages (from-to)2032-2044
Number of pages13
JournalJournal of Proteome Research
Volume8
Issue number4
DOIs
Publication statusPublished - 3 Apr 2009
Externally publishedYes

Keywords

  • 2D PAGE
  • CLIC1
  • Endometrium
  • Mass spectrometry
  • PGRMC1
  • Proliferative and secretory phases of the menstrual cycle
  • Proteome
  • Rho-GDIα
  • Two-dimensional differential in-gel electrophoresis (2D DIGE)

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