Proteomic and genomic characterization of highly infectious Clostridium difficile 630 spores

Trevor D. Lawley, Nicholas J. Croucher, Lu Yu, Simon Clare, Mohammed Sebaihia, David Goulding, Derek J. Pickard, Julian Parkhill, Jyoti Choudhary, Gordon Dougan

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151 Citations (Scopus)

Abstract

Clostridium difficile, a major cause of antibiotic-associated diarrhea, produces highly resistant spores that contaminate hospital environments and facilitate efficient disease transmission. We purified C. difficile spores using a novel method and show that they exhibit significant resistance to harsh physical or chemical treatments and are also highly infectious, with <7 environmental spores per cm2 reproducibly establishing a persistent infection in exposed mice. Mass spectrometric analysis identified ∼336 spore-associated polypeptides, with a significant proportion linked to translation, sporulation/germination, and protein stabilization/degradation. In addition, proteins from several distinct metabolic pathways associated with energy production were identified. Comparison of the C. difficile spore proteome to those of other clostridial species defined 88 proteins as the clostridial spore "core" and 29 proteins as C. difficile spore specific, including proteins that could contribute to spore-host interactions. Thus, our results provide the first molecular definition of C. difficile spores, opening up new opportunities for the development of diagnostic and therapeutic approaches.

Original languageEnglish
Pages (from-to)5377-5386
Number of pages10
JournalJournal of Bacteriology
Volume191
Issue number17
DOIs
Publication statusPublished - 1 Sep 2009
Externally publishedYes

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