Proteomic analysis on anti-proliferative and apoptosis effects of curcumin analog, 1,5-bis(4-hydroxy-3-methyoxyphenyl)-1,4-pentadiene-3-one-treated human glioblastoma and neuroblastoma cells

Yee Qian Lee, Pathmanathan Rajadurai, Faridah Abas, Iekhsan Othman, Rakesh Naidu

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    Abstract

    Curcumin analogs with excellent biological properties have been synthesized to address and overcome the poor pharmacokinetic profiles of curcumin. This study aims to investigate the cytotoxicity, anti-proliferative, and apoptosis-inducing ability of curcumin analog, MS13 on human glioblastoma U-87 MG, and neuroblastoma SH-SY5Y cells, and to examine the global proteome changes in these cells following treatment. Our current findings showed that MS13 induced potent cytotoxicity and anti-proliferative effects on both cells. Increased caspase-3 activity and decreased bcl-2 concentration upon treatment indicate that MS13 induces apoptosis in these cells in a dose- and time-dependent manner. The label-free shotgun proteomic analysis has defined the protein profiles in both glioblastoma and neuroblastoma cells, whereby a total of nine common DEPs, inclusive of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), alpha-enolase (ENO1), heat shock protein HSP 90-alpha (HSP90AA1), Heat shock protein HSP 90-beta (HSP90AB1), Eukaryotic translation initiation factor 5A-1 (EFI5A), heterogenous nuclear ribonucleoprotein K (HNRNPK), tubulin beta chain (TUBB), histone H2AX (H2AFX), and Protein SET were identified. Pathway analysis further elucidated that MS13 may induce its anti-tumor effects in both cells via the common enriched pathways, “Glycolysis” and “Post-translational protein modification.” Conclusively, MS13 demonstrates an anti-cancer effect that may indicate its potential use in the management of brain malignancies.

    Original languageEnglish
    Article number645856
    Number of pages16
    JournalFrontiers in Molecular Biosciences
    Volume8
    DOIs
    Publication statusPublished - 30 Apr 2021

    Keywords

    • anti-proliferation
    • apoptosis
    • cytotoxicity
    • diarylpentanoids
    • glioblastoma
    • neuroblastoma
    • shotgun proteomics

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