TY - JOUR
T1 - Proteomic analysis on anti-proliferative and apoptosis effects of curcumin analog, 1,5-bis(4-hydroxy-3-methyoxyphenyl)-1,4-pentadiene-3-one-treated human glioblastoma and neuroblastoma cells
AU - Lee, Yee Qian
AU - Rajadurai, Pathmanathan
AU - Abas, Faridah
AU - Othman, Iekhsan
AU - Naidu, Rakesh
N1 - Funding Information:
The authors would like to acknowledge the Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, for the research facilities and support and the LCMS/MS Platform, Monash University Malaysia, for processing the proteomic sample. YL would also like to acknowledge Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, for providing the scholarship. Funding. This research was funded by Fundamental Research Grant Scheme (FRGS/1/2016/SKK08/MUSM/02/1) under the Ministry of Education (MOE), Malaysia.
Publisher Copyright:
© Copyright © 2021 Lee, Rajadurai, Abas, Othman and Naidu.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/4/30
Y1 - 2021/4/30
N2 - Curcumin analogs with excellent biological properties have been synthesized to address and overcome the poor pharmacokinetic profiles of curcumin. This study aims to investigate the cytotoxicity, anti-proliferative, and apoptosis-inducing ability of curcumin analog, MS13 on human glioblastoma U-87 MG, and neuroblastoma SH-SY5Y cells, and to examine the global proteome changes in these cells following treatment. Our current findings showed that MS13 induced potent cytotoxicity and anti-proliferative effects on both cells. Increased caspase-3 activity and decreased bcl-2 concentration upon treatment indicate that MS13 induces apoptosis in these cells in a dose- and time-dependent manner. The label-free shotgun proteomic analysis has defined the protein profiles in both glioblastoma and neuroblastoma cells, whereby a total of nine common DEPs, inclusive of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), alpha-enolase (ENO1), heat shock protein HSP 90-alpha (HSP90AA1), Heat shock protein HSP 90-beta (HSP90AB1), Eukaryotic translation initiation factor 5A-1 (EFI5A), heterogenous nuclear ribonucleoprotein K (HNRNPK), tubulin beta chain (TUBB), histone H2AX (H2AFX), and Protein SET were identified. Pathway analysis further elucidated that MS13 may induce its anti-tumor effects in both cells via the common enriched pathways, “Glycolysis” and “Post-translational protein modification.” Conclusively, MS13 demonstrates an anti-cancer effect that may indicate its potential use in the management of brain malignancies.
AB - Curcumin analogs with excellent biological properties have been synthesized to address and overcome the poor pharmacokinetic profiles of curcumin. This study aims to investigate the cytotoxicity, anti-proliferative, and apoptosis-inducing ability of curcumin analog, MS13 on human glioblastoma U-87 MG, and neuroblastoma SH-SY5Y cells, and to examine the global proteome changes in these cells following treatment. Our current findings showed that MS13 induced potent cytotoxicity and anti-proliferative effects on both cells. Increased caspase-3 activity and decreased bcl-2 concentration upon treatment indicate that MS13 induces apoptosis in these cells in a dose- and time-dependent manner. The label-free shotgun proteomic analysis has defined the protein profiles in both glioblastoma and neuroblastoma cells, whereby a total of nine common DEPs, inclusive of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), alpha-enolase (ENO1), heat shock protein HSP 90-alpha (HSP90AA1), Heat shock protein HSP 90-beta (HSP90AB1), Eukaryotic translation initiation factor 5A-1 (EFI5A), heterogenous nuclear ribonucleoprotein K (HNRNPK), tubulin beta chain (TUBB), histone H2AX (H2AFX), and Protein SET were identified. Pathway analysis further elucidated that MS13 may induce its anti-tumor effects in both cells via the common enriched pathways, “Glycolysis” and “Post-translational protein modification.” Conclusively, MS13 demonstrates an anti-cancer effect that may indicate its potential use in the management of brain malignancies.
KW - anti-proliferation
KW - apoptosis
KW - cytotoxicity
KW - diarylpentanoids
KW - glioblastoma
KW - neuroblastoma
KW - shotgun proteomics
UR - http://www.scopus.com/inward/record.url?scp=85105945046&partnerID=8YFLogxK
U2 - 10.3389/fmolb.2021.645856
DO - 10.3389/fmolb.2021.645856
M3 - Article
C2 - 33996900
AN - SCOPUS:85105945046
SN - 2296-889X
VL - 8
JO - Frontiers in Molecular Biosciences
JF - Frontiers in Molecular Biosciences
M1 - 645856
ER -