Abstract
Didemnin B induces rapid apoptosis in human promyeloid HL-60 cells with an optimal concentration of 1 microM (Grubb et al. (1995) Biochem. Biophys. Res. Commum. 215, 1130-1136), but little is known about how it does so. In order to determine whether protein tyrosine phosphorylation is involved in this rapid induction of apoptosis, HL-60 cells were pre-treated with tyrosine kinase inhibitors for 1 h before didemnin B treatment. Genistein, 2,5-dihydroxycinnamic acid methyl ester, and a range of tyrphostins inhibit didemnin B-induced apoptotic morphology in a concentration-dependent manner. DNA fragmentation induced by didemnin B is also inhibited by genistein, 2,5-dihydroxycinnamic acid methyl ester, and tyrphostins.
| Original language | English |
|---|---|
| Pages (from-to) | 1 - 5 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 383 |
| Issue number | 1-2 |
| Publication status | Published - 1996 |
Cite this
Johnson, K. L., Vaillant, F., & Lawen, A. (1996). Protein tyrosine kinase inhibitors prevent didemnin B-induced apoptosis in HL-60 cells. FEBS Letters, 383(1-2), 1 - 5.