Abstract
Protein synthesis and secretion by post-natal sheep and calf retinal pigment epithelial (RPE) cells was investigated following labelling of choroidal pieces, isolated RPE cells and RPE cells in tissue culture with l-[U-14C] leucine. We show that RPE cells secrete a specific set of proteins that includes retinol binding protein (RBP) and transthyretin (TTR), which are both involved in retinol transport in blood. Using a two-chambered culture system we show that protein secretion by the post-natal RPE cells occurs predominantly across the apical pole of the cells, i.e., across the surface of the cells which, in vivo, faces the retina. In agreement with results of others using foetal RPE cells (Ong, D.E., Davis, J.T., O'Day, W.T. and Bok, D. (1994) Biochemistry 33, 1835-1842) we show that RBP and, to a lesser extent, TTR are also secreted predominantly across the apical pole of the cell. We have developed a cell culture model for the RPE that may be used as an in vitro model for studying transport across the blood-retinal barrier.
| Original language | English |
|---|---|
| Pages (from-to) | 121-129 |
| Number of pages | 9 |
| Journal | BBA - General Subjects |
| Volume | 1245 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 17 Aug 1995 |
Keywords
- Protein synthesis
- Retinal pigment epithelium
- Retinol
- Retinol binding protein
- Secretion
- Transthyretin
Cite this
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Protein synthesis and secretion by cultured retinal pigment epithelia. / Jaworowski, Anthony; Fang, Zhi Ping; Khong, Tee Fern; Augusteyn, Robert C.
In: BBA - General Subjects, Vol. 1245, No. 1, 17.08.1995, p. 121-129.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Protein synthesis and secretion by cultured retinal pigment epithelia
AU - Jaworowski, Anthony
AU - Fang, Zhi Ping
AU - Khong, Tee Fern
AU - Augusteyn, Robert C.
PY - 1995/8/17
Y1 - 1995/8/17
N2 - Protein synthesis and secretion by post-natal sheep and calf retinal pigment epithelial (RPE) cells was investigated following labelling of choroidal pieces, isolated RPE cells and RPE cells in tissue culture with l-[U-14C] leucine. We show that RPE cells secrete a specific set of proteins that includes retinol binding protein (RBP) and transthyretin (TTR), which are both involved in retinol transport in blood. Using a two-chambered culture system we show that protein secretion by the post-natal RPE cells occurs predominantly across the apical pole of the cells, i.e., across the surface of the cells which, in vivo, faces the retina. In agreement with results of others using foetal RPE cells (Ong, D.E., Davis, J.T., O'Day, W.T. and Bok, D. (1994) Biochemistry 33, 1835-1842) we show that RBP and, to a lesser extent, TTR are also secreted predominantly across the apical pole of the cell. We have developed a cell culture model for the RPE that may be used as an in vitro model for studying transport across the blood-retinal barrier.
AB - Protein synthesis and secretion by post-natal sheep and calf retinal pigment epithelial (RPE) cells was investigated following labelling of choroidal pieces, isolated RPE cells and RPE cells in tissue culture with l-[U-14C] leucine. We show that RPE cells secrete a specific set of proteins that includes retinol binding protein (RBP) and transthyretin (TTR), which are both involved in retinol transport in blood. Using a two-chambered culture system we show that protein secretion by the post-natal RPE cells occurs predominantly across the apical pole of the cells, i.e., across the surface of the cells which, in vivo, faces the retina. In agreement with results of others using foetal RPE cells (Ong, D.E., Davis, J.T., O'Day, W.T. and Bok, D. (1994) Biochemistry 33, 1835-1842) we show that RBP and, to a lesser extent, TTR are also secreted predominantly across the apical pole of the cell. We have developed a cell culture model for the RPE that may be used as an in vitro model for studying transport across the blood-retinal barrier.
KW - Protein synthesis
KW - Retinal pigment epithelium
KW - Retinol
KW - Retinol binding protein
KW - Secretion
KW - Transthyretin
UR - http://www.scopus.com/inward/record.url?scp=0029151115&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(95)00079-Q
DO - 10.1016/0304-4165(95)00079-Q
M3 - Article
VL - 1245
SP - 121
EP - 129
JO - Biochimica et Biophysica Acta. General Subjects
JF - Biochimica et Biophysica Acta. General Subjects
SN - 0304-4165
IS - 1
ER -