Protein separation using immobilized phospholipid chromatography

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Abstract

The chromatographic support contained monolayers of phospholipids offers novel modes in analyzing and separating proteins. The polar choline head groups on immobolized phosphatidylcholine were used for the affinity purification of phospholipase A (PLA). The purification process involves removing the contaminating proteins with detergent additives to the elution buffer such as short-chain alkylsulfonates. The lipid-bound PLA was eluted with acetonitrile or octyllysophosphatidylcholine. The purity of PLA was approximately 70 based on densitometric scans of gel electrophoresis. These results suggest that the lipid-immobolized chromatography may be applied to develop purification methods for PLA, enzymes, and membrane proteins obtained from diverse cells.
Original languageEnglish
Title of host publicationAffinity Chromatography - Methods and Protocols
EditorsM Zachariou
Place of PublicationTotowa NY USA
PublisherHumana Press
Pages295 - 302
Number of pages8
Edition2nd
ISBN (Print)978-1-58829-659-7
Publication statusPublished - 2008

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