Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

E H Abdelkader; A Feintuch; X Yao; L A Adams; L Aurelio; B Graham; D Goldfarb; G Otting

doi:10.1039/c5cc07121f

Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

E H Abdelkader, A Feintuch, X Yao, L A Adams, L Aurelio, B Graham, D Goldfarb, G Otting

Medicinal Chemistry

Research output: Contribution to journal › Article › Research › peer-review

66 Citations (Scopus)

Abstract

Quantitative cysteine-independent ligation of a Gd³⁺ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd³⁺-Gd³⁺ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd³⁺ ion relative to the protein can be predicted with high accuracy.

Original language	English
Pages (from-to)	15898-15901
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	88
DOIs	https://doi.org/10.1039/c5cc07121f
Publication status	Published - 2015

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10.1039/c5cc07121f

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title = "Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine",

abstract = "Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.",

author = "Abdelkader, {E H} and A Feintuch and X Yao and Adams, {L A} and L Aurelio and B Graham and D Goldfarb and G Otting",

year = "2015",

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journal = "Chemical Communications",

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publisher = "The Royal Society of Chemistry",

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T1 - Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

AU - Abdelkader, E H

AU - Feintuch, A

AU - Yao, X

AU - Adams, L A

AU - Aurelio, L

AU - Graham, B

AU - Goldfarb, D

AU - Otting, G

PY - 2015

Y1 - 2015

N2 - Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.

AB - Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.

UR - http://pubs.rsc.org.ezproxy.lib.monash.edu.au/en/content/articlepdf/2015/cc/c5cc07121f

U2 - 10.1039/c5cc07121f

DO - 10.1039/c5cc07121f

M3 - Article

SN - 1359-7345

VL - 51

SP - 15898

EP - 15901

JO - Chemical Communications

JF - Chemical Communications

IS - 88

ER -

Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

Abstract

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Expanding the molecular tool set for structural studies of proteins and their complexes

Cite this

Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

Abstract

Access to Document

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Projects

Expanding the molecular tool set for structural studies of proteins and their complexes

Cite this