Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

E H Abdelkader, A Feintuch, X Yao, L A Adams, L Aurelio, B Graham, D Goldfarb, G Otting

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.
Original languageEnglish
Pages (from-to)15898-15901
Number of pages4
JournalChemical Communications
Volume51
Issue number88
DOIs
Publication statusPublished - 2015

Cite this

Abdelkader, E H ; Feintuch, A ; Yao, X ; Adams, L A ; Aurelio, L ; Graham, B ; Goldfarb, D ; Otting, G. / Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine. In: Chemical Communications. 2015 ; Vol. 51, No. 88. pp. 15898-15901.
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abstract = "Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.",
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Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine. / Abdelkader, E H; Feintuch, A; Yao, X; Adams, L A; Aurelio, L; Graham, B; Goldfarb, D; Otting, G.

In: Chemical Communications, Vol. 51, No. 88, 2015, p. 15898-15901.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Abdelkader, E H

AU - Feintuch, A

AU - Yao, X

AU - Adams, L A

AU - Aurelio, L

AU - Graham, B

AU - Goldfarb, D

AU - Otting, G

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AB - Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.

UR - http://pubs.rsc.org.ezproxy.lib.monash.edu.au/en/content/articlepdf/2015/cc/c5cc07121f

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DO - 10.1039/c5cc07121f

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EP - 15901

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