Protein conformation by EPR spectroscopy using gadolinium tags clicked to genetically encoded p-azido-L-phenylalanine

E H Abdelkader, A Feintuch, X Yao, L A Adams, L Aurelio, B Graham, D Goldfarb, G Otting

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Abstract

Quantitative cysteine-independent ligation of a Gd3+ tag to genetically encoded p-azido-l-phenylalanine via Cu(i)-catalyzed click chemistry is shown to deliver an exceptionally powerful tool for Gd3+-Gd3+ distance measurements by double electron-electron resonance (DEER) experiments, as the position of the Gd3+ ion relative to the protein can be predicted with high accuracy.
Original languageEnglish
Pages (from-to)15898-15901
Number of pages4
JournalChemical Communications
Volume51
Issue number88
DOIs
Publication statusPublished - 2015

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