Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling

Daniel Philip Poole; Silvia Amadesi; Nicholas Andrew Veldhuis; Fe C Abogadie; Tina Marie Lieu; William Darby; Wolfgang Liedtke; Michael J Lew; Peter B McIntyre; Nigel William Bunnett

doi:10.1074/jbc.M112.438184

Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling

Daniel Philip Poole, Silvia Amadesi, Nicholas Andrew Veldhuis, Fe C Abogadie, Tina Marie Lieu, William Darby, Wolfgang Liedtke, Michael J Lew, Peter B McIntyre, Nigel William Bunnett

Drug Discovery Biology

Research output: Contribution to journal › Article › Research › peer-review

134 Citations (Scopus)

Abstract

Background: Receptors activate channels of sensory nerves to cause inflammation and pain by unknown mechanisms. Results: Protease-activated receptor 2 (PAR2) stimulated transient receptor potential vanilloid 4 (TRPV4) by generation of channel agonists. This required a key TRPV4 tyrosine and induced inflammation. Conclusion: PAR2 opens TRPV4 by functional coupling. Significance: Antagonism of PAR2-TRPV4 coupling could alleviate inflammation and pain.

Original language	English
Pages (from-to)	5790 - 5802
Number of pages	13
Journal	Journal of Biological Chemistry
Volume	288
Issue number	8
DOIs	https://doi.org/10.1074/jbc.M112.438184
Publication status	Published - 2013

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10.1074/jbc.M112.438184

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Poole, D. P., Amadesi, S., Veldhuis, N. A., Abogadie, F. C., Lieu, T. M., Darby, W., Liedtke, W., Lew, M. J., McIntyre, P. B., & Bunnett, N. W. (2013). Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling. Journal of Biological Chemistry, 288(8), 5790 - 5802. https://doi.org/10.1074/jbc.M112.438184

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title = "Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling",

abstract = "Background: Receptors activate channels of sensory nerves to cause inflammation and pain by unknown mechanisms. Results: Protease-activated receptor 2 (PAR2) stimulated transient receptor potential vanilloid 4 (TRPV4) by generation of channel agonists. This required a key TRPV4 tyrosine and induced inflammation. Conclusion: PAR2 opens TRPV4 by functional coupling. Significance: Antagonism of PAR2-TRPV4 coupling could alleviate inflammation and pain.",

author = "Poole, {Daniel Philip} and Silvia Amadesi and Veldhuis, {Nicholas Andrew} and Abogadie, {Fe C} and Lieu, {Tina Marie} and William Darby and Wolfgang Liedtke and Lew, {Michael J} and McIntyre, {Peter B} and Bunnett, {Nigel William}",

year = "2013",

doi = "10.1074/jbc.M112.438184",

language = "English",

volume = "288",

pages = "5790 -- 5802",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology",

number = "8",

}

Poole, DP, Amadesi, S, Veldhuis, NA, Abogadie, FC, Lieu, TM, Darby, W, Liedtke, W, Lew, MJ, McIntyre, PB & Bunnett, NW 2013, 'Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling', Journal of Biological Chemistry, vol. 288, no. 8, pp. 5790 - 5802. https://doi.org/10.1074/jbc.M112.438184

Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling. / Poole, Daniel Philip; Amadesi, Silvia; Veldhuis, Nicholas Andrew et al.
In: Journal of Biological Chemistry, Vol. 288, No. 8, 2013, p. 5790 - 5802.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling

AU - Poole, Daniel Philip

AU - Amadesi, Silvia

AU - Veldhuis, Nicholas Andrew

AU - Abogadie, Fe C

AU - Lieu, Tina Marie

AU - Darby, William

AU - Liedtke, Wolfgang

AU - Lew, Michael J

AU - McIntyre, Peter B

AU - Bunnett, Nigel William

PY - 2013

Y1 - 2013

N2 - Background: Receptors activate channels of sensory nerves to cause inflammation and pain by unknown mechanisms. Results: Protease-activated receptor 2 (PAR2) stimulated transient receptor potential vanilloid 4 (TRPV4) by generation of channel agonists. This required a key TRPV4 tyrosine and induced inflammation. Conclusion: PAR2 opens TRPV4 by functional coupling. Significance: Antagonism of PAR2-TRPV4 coupling could alleviate inflammation and pain.

AB - Background: Receptors activate channels of sensory nerves to cause inflammation and pain by unknown mechanisms. Results: Protease-activated receptor 2 (PAR2) stimulated transient receptor potential vanilloid 4 (TRPV4) by generation of channel agonists. This required a key TRPV4 tyrosine and induced inflammation. Conclusion: PAR2 opens TRPV4 by functional coupling. Significance: Antagonism of PAR2-TRPV4 coupling could alleviate inflammation and pain.

UR - http://www.jbc.org/content/288/8/5790

U2 - 10.1074/jbc.M112.438184

DO - 10.1074/jbc.M112.438184

M3 - Article

SN - 0021-9258

VL - 288

SP - 5790

EP - 5802

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 8

ER -

Protease-activated receptor 2 (PAR2) protein and transient receptor potential vanilloid 4 (TRPV4) protein coupling is required for sustained inflammatory signaling

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