Prospective evaluation of ResistancePlus MG, a new multiplex quantitative PCR assay for detection of Mycoplasma genitalium and macrolide resistance

S N Tabrizi, C. S. Bradshaw, C. K. Fairley, S. Walker, L. Y. Tan, Elisa Mokany

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Mycoplasma genitalium is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. As conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23S rRNA gene have been described to predict resistance. Recently, a novel multiplex quantitative PCR (qPCR) assay, ResistancePlus MG, has been described for the simultaneous detection of Mycoplasma genitalium and macrolide resistance. In the current study, the clinical performance of the assay was evaluated on 1,089 consecutive urine and anogenital swab samples in symptomatic and asymptomatic male and female patients. Overall, 6.0% were positive for M. genitalium, with 63.1% having macrolide resistance-associated mutations. Compared to the laboratory-validated qPCR method targeting the 16S rRNA gene and Sanger sequencing to determine 23S rRNA mutations, the sensitivity and specificity of M. genitalium detection were 98.5% and 100% and for detection of macrolide resistance mutations were 100.0% and 96.2%, respectively. This assay offers a considerable advantage in clinical settings for M. genitalium testing by making the results of macrolide resistance and mutation analyses simultaneously available, which is increasingly important with escalating macrolide resistance.

Original languageEnglish
Pages (from-to)1915-1919
Number of pages5
JournalJournal of Clinical Microbiology
Volume55
Issue number6
DOIs
Publication statusPublished - 1 Jun 2017

Keywords

  • 23S rRNA
  • Diagnostic
  • Macrolide resistance
  • Multiplex assay
  • Mycoplasma genitalium
  • NAAT
  • qPCR
  • Sensitivity and specificity

Cite this

@article{4b82018c14704d56a5fb205c933afc2f,
title = "Prospective evaluation of ResistancePlus MG, a new multiplex quantitative PCR assay for detection of Mycoplasma genitalium and macrolide resistance",
abstract = "Mycoplasma genitalium is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. As conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23S rRNA gene have been described to predict resistance. Recently, a novel multiplex quantitative PCR (qPCR) assay, ResistancePlus MG, has been described for the simultaneous detection of Mycoplasma genitalium and macrolide resistance. In the current study, the clinical performance of the assay was evaluated on 1,089 consecutive urine and anogenital swab samples in symptomatic and asymptomatic male and female patients. Overall, 6.0{\%} were positive for M. genitalium, with 63.1{\%} having macrolide resistance-associated mutations. Compared to the laboratory-validated qPCR method targeting the 16S rRNA gene and Sanger sequencing to determine 23S rRNA mutations, the sensitivity and specificity of M. genitalium detection were 98.5{\%} and 100{\%} and for detection of macrolide resistance mutations were 100.0{\%} and 96.2{\%}, respectively. This assay offers a considerable advantage in clinical settings for M. genitalium testing by making the results of macrolide resistance and mutation analyses simultaneously available, which is increasingly important with escalating macrolide resistance.",
keywords = "23S rRNA, Diagnostic, Macrolide resistance, Multiplex assay, Mycoplasma genitalium, NAAT, qPCR, Sensitivity and specificity",
author = "Tabrizi, {S N} and Bradshaw, {C. S.} and Fairley, {C. K.} and S. Walker and Tan, {L. Y.} and Elisa Mokany",
year = "2017",
month = "6",
day = "1",
doi = "10.1128/JCM.02312-16",
language = "English",
volume = "55",
pages = "1915--1919",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "6",

}

Prospective evaluation of ResistancePlus MG, a new multiplex quantitative PCR assay for detection of Mycoplasma genitalium and macrolide resistance. / Tabrizi, S N; Bradshaw, C. S.; Fairley, C. K.; Walker, S.; Tan, L. Y.; Mokany, Elisa.

In: Journal of Clinical Microbiology, Vol. 55, No. 6, 01.06.2017, p. 1915-1919.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Prospective evaluation of ResistancePlus MG, a new multiplex quantitative PCR assay for detection of Mycoplasma genitalium and macrolide resistance

AU - Tabrizi, S N

AU - Bradshaw, C. S.

AU - Fairley, C. K.

AU - Walker, S.

AU - Tan, L. Y.

AU - Mokany, Elisa

PY - 2017/6/1

Y1 - 2017/6/1

N2 - Mycoplasma genitalium is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. As conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23S rRNA gene have been described to predict resistance. Recently, a novel multiplex quantitative PCR (qPCR) assay, ResistancePlus MG, has been described for the simultaneous detection of Mycoplasma genitalium and macrolide resistance. In the current study, the clinical performance of the assay was evaluated on 1,089 consecutive urine and anogenital swab samples in symptomatic and asymptomatic male and female patients. Overall, 6.0% were positive for M. genitalium, with 63.1% having macrolide resistance-associated mutations. Compared to the laboratory-validated qPCR method targeting the 16S rRNA gene and Sanger sequencing to determine 23S rRNA mutations, the sensitivity and specificity of M. genitalium detection were 98.5% and 100% and for detection of macrolide resistance mutations were 100.0% and 96.2%, respectively. This assay offers a considerable advantage in clinical settings for M. genitalium testing by making the results of macrolide resistance and mutation analyses simultaneously available, which is increasingly important with escalating macrolide resistance.

AB - Mycoplasma genitalium is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. As conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23S rRNA gene have been described to predict resistance. Recently, a novel multiplex quantitative PCR (qPCR) assay, ResistancePlus MG, has been described for the simultaneous detection of Mycoplasma genitalium and macrolide resistance. In the current study, the clinical performance of the assay was evaluated on 1,089 consecutive urine and anogenital swab samples in symptomatic and asymptomatic male and female patients. Overall, 6.0% were positive for M. genitalium, with 63.1% having macrolide resistance-associated mutations. Compared to the laboratory-validated qPCR method targeting the 16S rRNA gene and Sanger sequencing to determine 23S rRNA mutations, the sensitivity and specificity of M. genitalium detection were 98.5% and 100% and for detection of macrolide resistance mutations were 100.0% and 96.2%, respectively. This assay offers a considerable advantage in clinical settings for M. genitalium testing by making the results of macrolide resistance and mutation analyses simultaneously available, which is increasingly important with escalating macrolide resistance.

KW - 23S rRNA

KW - Diagnostic

KW - Macrolide resistance

KW - Multiplex assay

KW - Mycoplasma genitalium

KW - NAAT

KW - qPCR

KW - Sensitivity and specificity

UR - http://www.scopus.com/inward/record.url?scp=85019611482&partnerID=8YFLogxK

U2 - 10.1128/JCM.02312-16

DO - 10.1128/JCM.02312-16

M3 - Article

VL - 55

SP - 1915

EP - 1919

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 6

ER -