Promoting extracellular matrix remodeling via ascorbic acid enhances the survival of primary ovarian follicles encapsulated in alginate hydrogels

David J Tagler; Yogeshwar Makanji; Tao Tu; Beatriz Penalver Bernabe; Raymond Lee; Jie Zhu; Ekaterina Kniazeva; Jessica E Hornick; Teresa K Woodruff; Lonnie D Shea

doi:10.1002/bit.25181

Promoting extracellular matrix remodeling via ascorbic acid enhances the survival of primary ovarian follicles encapsulated in alginate hydrogels

David J Tagler, Yogeshwar Makanji, Tao Tu, Beatriz Penalver Bernabe, Raymond Lee, Jie Zhu, Ekaterina Kniazeva, Jessica E Hornick, Teresa K Woodruff, Lonnie D Shea

Research output: Contribution to journal › Article › Other

56 Citations (Scopus)

Abstract

The in vitro growth of ovarian follicles is an emerging technology for fertility preservation. Various strategies support the culture of secondary and multilayer follicles from various species including mice, non-human primate, and human; however, the culture of early stage (primary and primordial) follicles, which are more abundant in the ovary and survive cryopreservation, has been limited. Hydrogel-encapsulating follicle culture systems that employed feeder cells, such as mouse embryonic fibroblasts (MEFs), stimulated the growth of primary follicles (70-80 microm); yet, survival was low and smaller follicles (

Original language	English
Pages (from-to)	1417 - 1429
Number of pages	13
Journal	Biotechnology and Bioengineering
Volume	111
Issue number	7
DOIs	https://doi.org/10.1002/bit.25181
Publication status	Published - 2014
Externally published	Yes

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Tagler, D. J., Makanji, Y., Tu, T., Bernabe, B. P., Lee, R., Zhu, J., Kniazeva, E., Hornick, J. E., Woodruff, T. K., & Shea, L. D. (2014). Promoting extracellular matrix remodeling via ascorbic acid enhances the survival of primary ovarian follicles encapsulated in alginate hydrogels. Biotechnology and Bioengineering, 111(7), 1417 - 1429. https://doi.org/10.1002/bit.25181

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abstract = "The in vitro growth of ovarian follicles is an emerging technology for fertility preservation. Various strategies support the culture of secondary and multilayer follicles from various species including mice, non-human primate, and human; however, the culture of early stage (primary and primordial) follicles, which are more abundant in the ovary and survive cryopreservation, has been limited. Hydrogel-encapsulating follicle culture systems that employed feeder cells, such as mouse embryonic fibroblasts (MEFs), stimulated the growth of primary follicles (70-80 microm); yet, survival was low and smaller follicles (",

author = "Tagler, {David J} and Yogeshwar Makanji and Tao Tu and Bernabe, {Beatriz Penalver} and Raymond Lee and Jie Zhu and Ekaterina Kniazeva and Hornick, {Jessica E} and Woodruff, {Teresa K} and Shea, {Lonnie D}",

year = "2014",

doi = "10.1002/bit.25181",

language = "English",

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AU - Tagler, David J

AU - Makanji, Yogeshwar

AU - Tu, Tao

AU - Bernabe, Beatriz Penalver

AU - Lee, Raymond

AU - Zhu, Jie

AU - Kniazeva, Ekaterina

AU - Hornick, Jessica E

AU - Woodruff, Teresa K

AU - Shea, Lonnie D

PY - 2014

Y1 - 2014

N2 - The in vitro growth of ovarian follicles is an emerging technology for fertility preservation. Various strategies support the culture of secondary and multilayer follicles from various species including mice, non-human primate, and human; however, the culture of early stage (primary and primordial) follicles, which are more abundant in the ovary and survive cryopreservation, has been limited. Hydrogel-encapsulating follicle culture systems that employed feeder cells, such as mouse embryonic fibroblasts (MEFs), stimulated the growth of primary follicles (70-80 microm); yet, survival was low and smaller follicles (

AB - The in vitro growth of ovarian follicles is an emerging technology for fertility preservation. Various strategies support the culture of secondary and multilayer follicles from various species including mice, non-human primate, and human; however, the culture of early stage (primary and primordial) follicles, which are more abundant in the ovary and survive cryopreservation, has been limited. Hydrogel-encapsulating follicle culture systems that employed feeder cells, such as mouse embryonic fibroblasts (MEFs), stimulated the growth of primary follicles (70-80 microm); yet, survival was low and smaller follicles (

UR - http://onlinelibrary.wiley.com/doi/10.1002/bit.25181/pdf

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DO - 10.1002/bit.25181

M3 - Article

SN - 0006-3592

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EP - 1429

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

IS - 7

ER -

Promoting extracellular matrix remodeling via ascorbic acid enhances the survival of primary ovarian follicles encapsulated in alginate hydrogels

Abstract

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