The hyperthermophilic archaeon Sulfolobus solfataricus metabolises glucose and galactose by a promiscuous non-phosphorylative variant of the Entner-Doudoroff pathway, in which a series of enzymes have sufficient substrate promiscuity to permit the metabolism of both sugars. Recently, it has been proposed that the part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus as an alternative route for glucose metabolism. In this report we demonstrate, by in vitro kinetic studies of D-2-keto-3-deoxygluconate (KDG) kinase and KDG aldolase, that the part-phosphorylative pathway in S. solfataricus is also promiscuous for the metabolism of both glucose and galactose.
|Pages (from-to)||6865 - 6869|
|Number of pages||5|
|Publication status||Published - 2005|
Lamble, H. J., Theodossis, A., Milburn, C. C., Taylor, G. L., Bull, S. D., Hough, D. W., & Danson, M. J. (2005). Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus. FEBS Letters, 579(30), 6865 - 6869.