Prolonged calcitonin receptor signaling by salmon, but not human calcitonin, reveals ligand bias

Kim Vietz Andreassen; Sara Toftegaard Hjuler; Sebastian G B Furness; Patrick M Sexton; Arthur Christopoulos; Olivier Nosjean; Morten Asser Karsdal; Kim Henriksen

doi:10.1371/journal.pone.0092042

Prolonged calcitonin receptor signaling by salmon, but not human calcitonin, reveals ligand bias

Kim Vietz Andreassen, Sara Toftegaard Hjuler, Sebastian G B Furness, Patrick M Sexton, Arthur Christopoulos, Olivier Nosjean, Morten Asser Karsdal, Kim Henriksen

Research output: Contribution to journal › Article › Research › peer-review

34 Citations (Scopus)

Abstract

Salmon calcitonin (sCT) and human calcitonin (hCT) are pharmacologically distinct. However, the reason for the differences is unclear. Here we analyze the differences between sCT and hCT on the human calcitonin receptor (CT(a)R) with respect to activation of cAMP signaling, beta-arrestin recruitment, ligand binding kinetics and internalization. The study was conducted using mammalian cell lines heterologously expressing the human CT(a) receptor. CT(a)R downstream signaling was investigated with dose response profiles for cAMP production and beta-arrestin recruitment for sCT and hCT during short term (

Original language	English
Pages (from-to)	1 - 10
Number of pages	10
Journal	PLoS ONE
Volume	9
Issue number	3 (Art. No: e92042)
DOIs	https://doi.org/10.1371/journal.pone.0092042
Publication status	Published - 2014

Cite this

Andreassen, K. V., Hjuler, S. T., Furness, S. G. B., Sexton, P. M., Christopoulos, A., Nosjean, O., Karsdal, M. A., & Henriksen, K. (2014). Prolonged calcitonin receptor signaling by salmon, but not human calcitonin, reveals ligand bias. PLoS ONE, 9(3 (Art. No: e92042)), 1 - 10. https://doi.org/10.1371/journal.pone.0092042

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Prolonged calcitonin receptor signaling by salmon, but not human calcitonin, reveals ligand bias. / Andreassen, Kim Vietz; Hjuler, Sara Toftegaard; Furness, Sebastian G B ; Sexton, Patrick M ; Christopoulos, Arthur; Nosjean, Olivier; Karsdal, Morten Asser; Henriksen, Kim.

In: PLoS ONE, Vol. 9, No. 3 (Art. No: e92042), 2014, p. 1 - 10.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Karsdal, Morten Asser

AU - Henriksen, Kim

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AB - Salmon calcitonin (sCT) and human calcitonin (hCT) are pharmacologically distinct. However, the reason for the differences is unclear. Here we analyze the differences between sCT and hCT on the human calcitonin receptor (CT(a)R) with respect to activation of cAMP signaling, beta-arrestin recruitment, ligand binding kinetics and internalization. The study was conducted using mammalian cell lines heterologously expressing the human CT(a) receptor. CT(a)R downstream signaling was investigated with dose response profiles for cAMP production and beta-arrestin recruitment for sCT and hCT during short term (

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