Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats

Maarten P Koeners; Connie P.C. Ow; David M Russell; Roger G Evans; Simon C Malpas

doi:10.1007/978-1-4939-3353-2_9

Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats

Maarten P Koeners, Connie P.C. Ow, David M Russell, Roger G Evans, Simon C Malpas

Physiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

13 Citations (Scopus)

Abstract

A relative deficiency in kidney oxygenation, i.e., renal hypoxia, may contribute to the initiation and progression of acute and chronic kidney disease. A critical barrier to investigate this is the lack of methods allowing measurement of the partial pressure of oxygen in kidney tissue for long periods in vivo. We have developed, validated, and tested a novel telemetric method that can do this. Here we provide details on the calibration, implantation, implementation for data recording, and reuse of this telemetry-based technology for measurement of medullary tissue oxygen tension in conscious, unrestrained rats. This technique provides an important additional tool for investigating the impact of renal hypoxia in biology and pathophysiology.

Original language	English
Title of host publication	Kidney Research
Subtitle of host publication	Experimental Protocols
Editors	T D Hewitson, E R Smith, S G Holt
Place of Publication	New York NY USA
Publisher	Humana Press
Pages	93-111
Number of pages	19
Edition	2nd
ISBN (Electronic)	9781493933532
ISBN (Print)	9781493933518
DOIs	https://doi.org/10.1007/978-1-4939-3353-2_9
Publication status	Published - 2016

Publication series

Name	Methods in Molecular Biology: Springer Protocols
Publisher	Springer
Volume	1397
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

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10.1007/978-1-4939-3353-2_9

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Koeners, M. P., Ow, C. P. C., Russell, D. M., Evans, R. G., & Malpas, S. C. (2016). Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats. In T. D. Hewitson, E. R. Smith, & S. G. Holt (Eds.), Kidney Research: Experimental Protocols (2nd ed., pp. 93-111). (Methods in Molecular Biology: Springer Protocols; Vol. 1397). Humana Press. https://doi.org/10.1007/978-1-4939-3353-2_9

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title = "Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats",

abstract = "A relative deficiency in kidney oxygenation, i.e., renal hypoxia, may contribute to the initiation and progression of acute and chronic kidney disease. A critical barrier to investigate this is the lack of methods allowing measurement of the partial pressure of oxygen in kidney tissue for long periods in vivo. We have developed, validated, and tested a novel telemetric method that can do this. Here we provide details on the calibration, implantation, implementation for data recording, and reuse of this telemetry-based technology for measurement of medullary tissue oxygen tension in conscious, unrestrained rats. This technique provides an important additional tool for investigating the impact of renal hypoxia in biology and pathophysiology.",

author = "Koeners, {Maarten P} and Ow, {Connie P.C.} and Russell, {David M} and Evans, {Roger G} and Malpas, {Simon C}",

year = "2016",

doi = "10.1007/978-1-4939-3353-2_9",

language = "English",

isbn = "9781493933518",

series = "Methods in Molecular Biology: Springer Protocols",

publisher = "Humana Press",

pages = "93--111",

editor = "Hewitson, {T D} and Smith, {E R} and Holt, {S G}",

booktitle = "Kidney Research",

address = "United States of America",

edition = "2nd",

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Koeners, MP, Ow, CPC, Russell, DM, Evans, RG & Malpas, SC 2016, Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats. in TD Hewitson, ER Smith & SG Holt (eds), Kidney Research: Experimental Protocols. 2nd edn, Methods in Molecular Biology: Springer Protocols, vol. 1397, Humana Press, New York NY USA, pp. 93-111. https://doi.org/10.1007/978-1-4939-3353-2_9

Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats. / Koeners, Maarten P; Ow, Connie P.C.; Russell, David M et al.
Kidney Research: Experimental Protocols. ed. / T D Hewitson; E R Smith; S G Holt. 2nd. ed. New York NY USA: Humana Press, 2016. p. 93-111 (Methods in Molecular Biology: Springer Protocols; Vol. 1397).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

TY - CHAP

T1 - Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats

AU - Koeners, Maarten P

AU - Ow, Connie P.C.

AU - Russell, David M

AU - Evans, Roger G

AU - Malpas, Simon C

PY - 2016

Y1 - 2016

N2 - A relative deficiency in kidney oxygenation, i.e., renal hypoxia, may contribute to the initiation and progression of acute and chronic kidney disease. A critical barrier to investigate this is the lack of methods allowing measurement of the partial pressure of oxygen in kidney tissue for long periods in vivo. We have developed, validated, and tested a novel telemetric method that can do this. Here we provide details on the calibration, implantation, implementation for data recording, and reuse of this telemetry-based technology for measurement of medullary tissue oxygen tension in conscious, unrestrained rats. This technique provides an important additional tool for investigating the impact of renal hypoxia in biology and pathophysiology.

AB - A relative deficiency in kidney oxygenation, i.e., renal hypoxia, may contribute to the initiation and progression of acute and chronic kidney disease. A critical barrier to investigate this is the lack of methods allowing measurement of the partial pressure of oxygen in kidney tissue for long periods in vivo. We have developed, validated, and tested a novel telemetric method that can do this. Here we provide details on the calibration, implantation, implementation for data recording, and reuse of this telemetry-based technology for measurement of medullary tissue oxygen tension in conscious, unrestrained rats. This technique provides an important additional tool for investigating the impact of renal hypoxia in biology and pathophysiology.

UR - http://www.ncbi.nlm.nih.gov/pubmed/26676130

U2 - 10.1007/978-1-4939-3353-2_9

DO - 10.1007/978-1-4939-3353-2_9

M3 - Chapter (Book)

SN - 9781493933518

T3 - Methods in Molecular Biology: Springer Protocols

SP - 93

EP - 111

BT - Kidney Research

A2 - Hewitson, T D

A2 - Smith, E R

A2 - Holt, S G

PB - Humana Press

CY - New York NY USA

ER -

Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats

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